The measurement of the phosphorescence and singlet oxygen fluorescence time-resolved waveforms of Photofrin(II) and Talaporfin sodium with pulsed excitation

Shiho Hakomori; Sayaka Ohmori; Kensuke Masuda; Kojiro Yamamoto; Tsunenori Arai

doi:10.1117/12.699857

28 February 2007 The measurement of the phosphorescence and singlet oxygen fluorescence time-resolved waveforms of Photofrin(II) and Talaporfin sodium with pulsed excitation

Shiho Hakomori, Sayaka Ohmori, Kensuke Masuda, Kojiro Yamamoto, Tsunenori Arai

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Proceedings Volume 6427, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XVI; 642715 (2007) https://doi.org/10.1117/12.699857
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

In order to investigate the interaction between the triplet state T₁ and ground state oxygen ³O₂ during pulsed excitation photodynamic therapy (PDT), we measured the phosphorescence and singlet oxygen ¹O₂ fluorescence time-resolved waveform. The phosphorescence time-resolved waveform from clinical photosensitizers has not been obtained because this signal was buried in the photosensitizer fluorescence. We constructed the experimental setup with a spectral and temporal filter to select the phosphorescence signals from the Photofrin(II)^(R) and Talaporfin sodium solution. The lifetimes and spectrums of the measured luminescence coincided with the phosphorescence characteristics, respectively. We obtained the phosphorescence time-resolved waveforms from the clinical photosensitizer solutions successfully. The ¹O₂ fluorescence time-resolved waveforms from these photosensitizers were measured with an IR-PMT with a photon counter. The fluorescence time-resolved waveforms of each photosensitizer were also obtained by the authors. We could consequently describe sequential generation of three time-resolved waveforms throughout the photosensitive reaction in the clinical photosensitizers. We think we may evaluate the photoseisitizer characteristics by these waveforms.

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Shiho Hakomori, Sayaka Ohmori, Kensuke Masuda, Kojiro Yamamoto, and Tsunenori Arai "The measurement of the phosphorescence and singlet oxygen fluorescence time-resolved waveforms of Photofrin(II) and Talaporfin sodium with pulsed excitation", Proc. SPIE 6427, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XVI, 642715 (28 February 2007); https://doi.org/10.1117/12.699857

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KEYWORDS

Phosphorescence

Luminescence

Sodium

Photodynamic therapy

Oxygen

Photonics

Camera shutters

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