Antioxidant assay using genetically engineered bioluminescent Escherichia coli

Amelita Bartolome; Bernadette Macalino; Ian Lemuel Pastoral; Fortunato Sevilla III

doi:10.1117/12.643950

23 February 2006 Antioxidant assay using genetically engineered bioluminescent Escherichia coli

Amelita Bartolome, Bernadette Macalino, Ian Lemuel Pastoral, Fortunato Sevilla III

Proceedings Volume 6098, Genetically Engineered Probes for Biomedical Applications; 60980J (2006) https://doi.org/10.1117/12.643950
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

A new antioxidant activity assay based on the reactive oxygen species (ROS)-inducible bacterial strain (E. coli DPD2511) is described. The strain harbors the plasmid pKatG::luxCDABE and responds to hydrogen peroxide treatment by increasing light emission at 490 nm. Antioxidant capacity is evaluated through the ability of an agent to inhibit the hydrogen peroxide-induced bioluminescence of E. coli DPD2511. Applicability of the developed assay in detecting levels of antioxidants in various aqueous plant extracts is demonstrated. The assay was validated against 2,2-diphenylpicrylhydrazyl (DPPH) assay, a known antioxidant assay.

Citation Download Citation

Amelita Bartolome, Bernadette Macalino, Ian Lemuel Pastoral, and Fortunato Sevilla III "Antioxidant assay using genetically engineered bioluminescent Escherichia coli", Proc. SPIE 6098, Genetically Engineered Probes for Biomedical Applications, 60980J (23 February 2006); https://doi.org/10.1117/12.643950

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