Study of thermal fluctuations during thermal denaturation of DNA

K. S. Nagapriya; Arup Kumar Raychaudhuri; Dipankar Chatterji

doi:10.1117/12.609411

23 May 2005 Study of thermal fluctuations during thermal denaturation of DNA

K. S. Nagapriya, Arup Kumar Raychaudhuri, Dipankar Chatterji

Proceedings Volume 5841, Fluctuations and Noise in Biological, Biophysical, and Biomedical Systems III; (2005) https://doi.org/10.1117/12.609411
Event: SPIE Third International Symposium on Fluctuations and Noise, 2005, Austin, Texas, United States

Abstract

In this paper we address the fundamental issue of temperature fluctuation during the thermal denaturation (or the unzipping of the two strands on heating) of double stranded (ds) DNA. From our experiments we observe the presence of extremely high thermal fluctuations during DNA denaturation. This thermal fluctuation is several orders higher than the thermal fluctuation at temperatures away from the denaturation temperature range. This fluctuation is absent in single stranded (ss) DNA. The magnitude of fluctuation is much higher in heteropolymeric DNA and is almost absent in short homopolymeric DNA fragments. The temperature range over which the denaturation occurs (i.e., over which the thermal fluctuation is large) depends on the length of the DNA and is largest for the longest DNA.

Citation Download Citation

K. S. Nagapriya, Arup Kumar Raychaudhuri, and Dipankar Chatterji "Study of thermal fluctuations during thermal denaturation of DNA", Proc. SPIE 5841, Fluctuations and Noise in Biological, Biophysical, and Biomedical Systems III, (23 May 2005); https://doi.org/10.1117/12.609411

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