Fluorescence enhancement near metallic nanoparticles under two photon excitation

Sandrine Leveque-Fort; Emmanuel Fort; Eric Le Moal; Jean Pierre Lacharme; Christian Ricolleau; Marie-Pierre Fontaine-Aupart

doi:10.1117/12.500655

9 October 2003 Fluorescence enhancement near metallic nanoparticles under two photon excitation

Sandrine Leveque-Fort, Emmanuel Fort, Eric Le Moal, Jean Pierre Lacharme, Christian Ricolleau, Marie-Pierre Fontaine-Aupart

Author Affiliations +

Proceedings Volume 5139, Confocal, Multiphoton, and Nonlinear Microscopic Imaging; (2003) https://doi.org/10.1117/12.500655
Event: European Conferences on Biomedical Optics 2003, 2003, Munich, Germany

Abstract

Fluorescence is widely used as a spectroscopic tool or for biomedical imaging. To extend these measurements to small concentrations or to fluorophores with very low quantum yield we have developed nanostructured substrates made of silver nanoparticles covered with a spacerlayer of alumina. Factors of about 200 are obtained for fluorescence enhancement with two photon excitation. Lifetime measurements reveal additional information on the decay channels induced by the nanoparticle presence.

Citation Download Citation

Sandrine Leveque-Fort, Emmanuel Fort, Eric Le Moal, Jean Pierre Lacharme, Christian Ricolleau, and Marie-Pierre Fontaine-Aupart "Fluorescence enhancement near metallic nanoparticles under two photon excitation", Proc. SPIE 5139, Confocal, Multiphoton, and Nonlinear Microscopic Imaging, (9 October 2003); https://doi.org/10.1117/12.500655

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