|
The salutary effect of laser therapy in medical practice connects with the improvement of microcirculation and the activation of cell proliferation. In the present paper we presented the experimental evidence for the concept of free radical mechanism of stimulating effect of the low level laser irradiation(LLLI). Polymorphonuclear leukocytes (PMNL) were obtained from patients with broncho-pulinonary and oncological diseases. PMNL were incubated at presence and in the absence of autologous plasma containing endogenous (porphyrins) and exogenous (phthalocyanines) photosensitizers. Then, cell suspension were irradiated using LLLI in the red (He-Ne-laser, ATML-l, wavelength -632,8 nm, Russia) and the infrared (UZOR, wavelength - 890 nm, "Electronics", Russia) ranges of spectrum. The content of porphyrins in autological plasma samples, the determination of lipid peroxidation products (TBAreactive products) in cells and the detection of intracellular calcium concentration in PMNL using FURA were estimated by spectrofluorimetric methods. It was found that the LLLI in red range of spectrum induced leukocyte priming in vitro. These effect was dose-dependent and accompanied by the increase of intracellular calcium concentration. During laser irradiation of leukocyte suspension at presence of autologous plasma was established that the priming index correlated with concentration of endogenous porphyrins and phthalocyanines in plasma samples. It was shown that the laser4nduced leukocyte priming depends not only on total lipid peroxide products in cell membranes but also its qualitative chemical composition. For the first time we have been found the LLLl4nduced leukocyte priming using infrared irradiation. We propose that the photo-induced free radical reactions underlie in the laser therapy.
|
