Fluorescence spectral imaging of organelle interaction

Elli Kohen; Joseph G. Hirschberg; Cahide Kohen; Dietrich O. Schachtschabel; Marco Monti; Rita Stanikunaite

doi:10.1117/12.384215

27 April 2000 Fluorescence spectral imaging of organelle interaction

Elli Kohen, Joseph G. Hirschberg, Cahide Kohen, Dietrich O. Schachtschabel, Marco Monti, Rita Stanikunaite

Proceedings Volume 3921, Optical Diagnostics of Living Cells III; (2000) https://doi.org/10.1117/12.384215
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

In cell biology, one of the great mysteries, which has bene only superficially 8investigate,d is the integration of cytoplasmic and nuclear organelles as part of the intracellular regulatory mechanism. The methodology used for the exploration of such intracellular processes is the pixel-by-pixel scanning by means of fluorescence spectral imaging and excitation emission fluorescence spectroscopy. While several of the steps required by this method are still in the process of implementation, the Michelson interferometer, the Sagnac interferometer and the related 'pentaferometer' are possible components of the instrumental design. One of the illustrative experimental models to begin the study of intracellular integrative processes is based on the hypothesis of a 'nuclear pump' in conjunction with cell treatment by chemotherapeutic agents such as adriamycin. Preliminary observations initiated in cultured fibroblasts, and to be pursued in Cloudman's melanoma cells, suggest that this cytotoxic agent first moves into the nucleus, form which it is subsequently ejected to be incorporated into the lysosomes and Golgi apparatus, possibly prior to exclusion via the multiple drug resistance pathway. The timetable of such a process is under investigation. This subject has obvious implications for diagnostic, prognostic and therapeutic studies of organelles integration.

Citation Download Citation

Elli Kohen, Joseph G. Hirschberg, Cahide Kohen, Dietrich O. Schachtschabel, Marco Monti, and Rita Stanikunaite "Fluorescence spectral imaging of organelle interaction", Proc. SPIE 3921, Optical Diagnostics of Living Cells III, (27 April 2000); https://doi.org/10.1117/12.384215

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