Fluorescence detection of the anticancer drug topotecan and related camptothecins in plasma and whole blood by two-photon excitation

Thomas G. Burke; Henryk M. Malak; Ignacy Gryczynski; Joseph R. Lakowicz

doi:10.1117/12.273532

7 May 1997 Fluorescence detection of the anticancer drug topotecan and related camptothecins in plasma and whole blood by two-photon excitation

Thomas G. Burke, Henryk M. Malak, Ignacy Gryczynski, Joseph R. Lakowicz

Author Affiliations +

Proceedings Volume 2980, Advances in Fluorescence Sensing Technology III; (1997) https://doi.org/10.1117/12.273532
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

Recent FDA-approval of topotecan (9-dimethylaminomethyl-10- hydroxycamptothecin) and camptosar (CPT-11) along with the accelerated clinical development of related camptothecin drugs provides new hope for the successful treatment of human cancer, including neoplasms for which no effective treatments currently exist. Current clinical efforts worldwide are aimed at optimizing the therapeutic efficacies of the camptothecins, with the major focus on the determination of the most effective dosing schedules. To this end, technological advances which provide a direct and rapid means of measuring plasma drug levels (i.e. such that correlations between plasma drug levels and clinical responses can be sought) would be of great utility. Here we report on the direct fluorescence detection of topotecan and SN-38 in human plasma and topotecan in whole blood at micro molar levels using two-photon excitation at 730 or 820 nm. Topotecan was detected at concentrations as low as 0.05 and 1 (mu) M in plasma and whole blood, respectively. Since skin, blood and other tissues are translucent at long wavelengths, our results suggest the attractive possibility of homogeneous or noninvasive clinical sensing of camptothecins in situ using two-photon excitation.

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Thomas G. Burke, Henryk M. Malak, Ignacy Gryczynski, and Joseph R. Lakowicz "Fluorescence detection of the anticancer drug topotecan and related camptothecins in plasma and whole blood by two-photon excitation", Proc. SPIE 2980, Advances in Fluorescence Sensing Technology III, (7 May 1997); https://doi.org/10.1117/12.273532

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KEYWORDS

Plasma

Blood

Luminescence

Fluorescence spectroscopy

Cancer

Oncology

Tissues

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