Laser capture microdissection to study flower morphogenesis

Magdalena Ewa Pawełkowicz; Agnieszka Skarzyńska; Cezary Kowalczuk; Wojciech Pląder; Zbigniew Przybecki

doi:10.1117/12.2280776

7 August 2017 Laser capture microdissection to study flower morphogenesis

Magdalena Ewa Pawełkowicz, Agnieszka Skarzyńska, Cezary Kowalczuk, Wojciech Pląder, Zbigniew Przybecki

Proceedings Volume 10445, Photonics Applications in Astronomy, Communications, Industry, and High Energy Physics Experiments 2017; 1044539 (2017) https://doi.org/10.1117/12.2280776
Event: Photonics Applications in Astronomy, Communications, Industry, and High-Energy Physics Experiments 2017, 2017, Wilga, Poland

Abstract

Laser Capture Microdissection (LCM) is a sample preparation microscopic method that enables isolation of an interesting cell or cells population from human, animal or plant tissue. This technique allows for obtaining pure sample from heterogeneous mixture. From isolated cells, it is possible to obtain the appropriate quality material used for genomic research in transcriptomics, proteomics and metabolomics. We used LCM method to study flower morphogenesis and specific bud’s organ organization and development. The genes expression level in developing flower buds of male (B10) and female (2gg) lines were analyzed with qPCR. The expression was checked for stamen and carpel primordia obtained with LCM and for whole flower buds at successive stages of growth.

Citation Download Citation

Magdalena Ewa Pawełkowicz, Agnieszka Skarzyńska, Cezary Kowalczuk, Wojciech Pląder, and Zbigniew Przybecki "Laser capture microdissection to study flower morphogenesis", Proc. SPIE 10445, Photonics Applications in Astronomy, Communications, Industry, and High Energy Physics Experiments 2017, 1044539 (7 August 2017); https://doi.org/10.1117/12.2280776

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