Cellular response of ovarian carcinoma cells to antibody-photosensitizer-mediated injury

Tayyaba Hasan; M. E. Sherwood; T. Anderson; Mike Bamberg; Thomas J. Flotte M.D.; Vince R. Zurawski Jr.

doi:10.1117/12.17657

1 July 1990 Cellular response of ovarian carcinoma cells to antibody-photosensitizer-mediated injury

Tayyaba Hasan, M. E. Sherwood, T. Anderson, Mike Bamberg, Thomas J. Flotte M.D., Vince R. Zurawski Jr.

Proceedings Volume 1203, Photodynamic Therapy: Mechanisms II; (1990) https://doi.org/10.1117/12.17657
Event: OE/LASE '90, 1990, Los Angeles, CA, United States

Abstract

An anti-ovarian carcinoma antibody OC125 was conjugated to a derivative of the photosensitizer (PS) chiorin e6 yj polyglutamic acid. Target cells from a human ovarian cancer cell line were treated with this conjugate and laser irradiation at 656 rim (absorption maximum of PS) and fixed 24 h later for electron microscopy. Electron niicrographs showed a high degree of vacoulization, generalized cell necrosis, and extrusion of organelles. No specific damage to the plasma membrane was noted. Untreated control cells, or cells treated with conjugate or light alone exhibited no injury. These data suggest that even though the antibody recognizes a cell surface antigen, the conjugate is internalized under the conditions of the experiment.

Citation Download Citation

Tayyaba Hasan, M. E. Sherwood, T. Anderson, Mike Bamberg, Thomas J. Flotte M.D., and Vince R. Zurawski Jr. "Cellular response of ovarian carcinoma cells to antibody-photosensitizer-mediated injury", Proc. SPIE 1203, Photodynamic Therapy: Mechanisms II, (1 July 1990); https://doi.org/10.1117/12.17657

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