Multidimensional microscopy image processing

Raj S. Acharya; Jagath K. Samarabandu; Ping Chin Cheng

doi:10.1117/12.146729

24 June 1993 Multidimensional microscopy image processing

Raj S. Acharya, Jagath K. Samarabandu, Ping Chin Cheng

Proceedings Volume 1895, Ultrasensitive Laboratory Diagnostics; (1993) https://doi.org/10.1117/12.146729
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

A confocal image processing system is developed for automatic identification (recognition) and characterization of confocal fluorescent images (serial optical sections). The system is capable of identifying a large percentage of structures (e.g., DNA replication sites) in the presence of background noise and non specific staining of cellular structures. A combination of image processing techniques are applied to successively refine the input image and is so organized as to find the surfaces of highly visible structures first, using simple image processing techniques, and then to adjust and fill in the missing areas of these object surfaces using a number of more complex image processing techniques. As a result, the image analysis system is capable of obtaining morphometric parameters such as surface area, volume, and position of structures of interest automatically. The system provides a powerful tool for biomedical research such as micro-structure characterization, morphogenesis, cell differentiation, tissue organization, and embryo development. We illustrate the performance of the confocal image analysis system by using an image of DNA replication sites in a mammalian 3T3 cell.

Citation Download Citation

Raj S. Acharya, Jagath K. Samarabandu, and Ping Chin Cheng "Multidimensional microscopy image processing", Proc. SPIE 1895, Ultrasensitive Laboratory Diagnostics, (24 June 1993); https://doi.org/10.1117/12.146729

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available