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Kinetic Measurements of Cell Surface E-Selectin/Carbohydrate Ligand Interactions

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Abstract

Selectin/ligand interactions initiate the multistep adhesion and signaling cascades in the recruitment of leukocytes from circulation to inflamed tissues and may also play a role in tumor metastasis. Kinetic properties of these interactions are essential determinants governing blood-borne cells' tethering to and rolling on the vessel wall. Extending our recently developed micropipette method, we have measured the kinetic rates of E-selectin/ligand interactions. Red cells coated with an E-selectin construct were allowed to bind HL-60 or Colo-205 cells bearing carbohydrate ligands. Specific adhesions were observed to occur at isolated points, the frequency of which followed a Poisson distribution. These point attachments were formed at the same rate with both the HL-60 and Colo-205 cells (0.14 ± 0.04 and 0.13 ± 0.03 µ m2s-1 per unit density of E-selectin, respectively) but dissociated from the former at a rate twice as fast as did from the latter (0.92 ± 0.23 and 0.44 ± 0.10s-1, respectively). The reverse rates agree well with those measured by the flow chamber. The forward rates are orders of magnitude higher than those of Fcγ receptors interacting with IgG measured under similar conditions, consistent with the rapid kinetics requirement for the function of E-selectin/ligand binding, which is to capture leukocytes on endothelial surfaces from flow. © 2001 Biomedical Engineering Society.

PAC2001: 8717-d, 8719Tt

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Authors and Affiliations

  1. George W. Woodruff School of Mechanical Engineering and Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA

    Mian Long, Hong Zhao & Cheng Zhu

  2. College of Bioengineering, Chongqing University, Chongqing, 400044

    Mian Long

  3. National Microgravity Laboratory, Institute of Mechanics, Chinese Academy of Science, Beijing, 100080, People's Republic of China

    Mian Long

  4. Department of Discovery Technologies, Hoffmann-La Roche Inc., Nutley, NJ

    Kuo-Sen Huang

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Long, M., Zhao, H., Huang, KS. et al. Kinetic Measurements of Cell Surface E-Selectin/Carbohydrate Ligand Interactions. Annals of Biomedical Engineering 29, 935–946 (2001). https://doi.org/10.1114/1.1415529

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