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Increased levels of a particular phosphatidylcholine species in senescent human dermal fibroblasts in vitro

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Abstract

Plasma membranes are essential components of living cells, and phospholipids are major components of cellular membranes. Here, we used liquid chromatography/mass spectrometry to investigate changes in the membrane phospholipid content that occur in association with aging. Our results indicate that the levels of a particular species of phosphatidylcholine comprised of stearic acid and arachidonic acid increased with age. To determine the reason forthe increased levels of this particular phosphatidylcholine, we examined the effect of highly unsaturated fatty acids, such as arachidonic acid and eicosapentaenoic acid, on cellular aging. Applied arachidonic acid was incorporated into phosphatidylcholine molecules, but neither arachidonic acid nor other related unsaturated fatty acids had any effect. We conclude that increased levels of this distinctive phosphatidylcholine are a result of in vitro senescence.

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Authors and Affiliations

  1. Research and Development Division, KOSÉ Corporation, 48-18 Sakae-cho, Kita-ku, 114-0005, Tokyo, Japan

    Eiji Naru, Misako Kobayashi, Yuko Misaki & Kumi Arakane

  2. Graduate School of Pharmaceutical Sciences, the University of Tokyo, Tokyo

    Yasukazu Takanezawa

  3. Laboratory of Cell and Molecular Biology of Aging, Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, Shizuoka, Japan

    Kazuhiko Kaji

Authors
  1. Eiji Naru
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  2. Yasukazu Takanezawa
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  3. Misako Kobayashi
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  4. Yuko Misaki
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  5. Kazuhiko Kaji
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  6. Kumi Arakane
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Correspondence to Eiji Naru.

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Naru, E., Takanezawa, Y., Kobayashi, M. et al. Increased levels of a particular phosphatidylcholine species in senescent human dermal fibroblasts in vitro . Hum Cell 21, 70–78 (2008). https://doi.org/10.1111/j.1749-0774.2008.00052.x

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  • DOI: https://doi.org/10.1111/j.1749-0774.2008.00052.x

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