Elsevier

Differentiation

Volume 28, Issue 3, March 1985, Pages 260-267
Differentiation

Serological analysis of early mouse embryo with rat monoclonal antibodies produced against mouse teratocarcinoma cells

https://doi.org/10.1111/j.1432-0436.1985.tb00834.xGet rights and content

Abstract

Rat-mouse hybridoma antibodies were produced against mouse teratocarcinoma F9 or PCC4 azal cells, and four clones were established. Both the Fll (IgM) and F20 (IgG2c) antibodies showed a similar specificity, reacting only with nullipotential teratocarcinoma cells. They were also found to agglutinate sheep red blood cells. Solid-phase enzyme-linked immunofluorescence assay showed that, among the neutral glycolipids studied, they only reacted with the Forssman antigen. P2 antibody (IgG2b) reacted with the undifferentiated-type and embryonal endodermtype teratocarcinoma cells. During the preimplantation stage, this antibody did not stain mouse embryos, but it reacted very weakly with the inner cell mass of blastocysts cultured in vitro. In the 5th-day embryo, the embryonic ectoderm as well as the visceral and parietal endoderm were positive, but the extraembryonic ectoderm was not. Mesoderm of the 7.5th-day embryo also reacted with this antibody. However, P2 antigen was not observed in the 16th-day embryo or in adult tissues. F2 antibody (IgG2a), which was reactive with all of the cultured cell lines tested, showed an immunoreaction with mouse embryos throughout the preimplantation stage. However, in the 7.5th-day embryo, the presence of F2 was limited to the cells forming the parietal endoderm. This antigen was present in some epithelial tissues of the 16th-day embryo and adult mouse. Of these antigens, P2 and F2 are probably novel differentiation antigens of the early mouse embryo. Together with the Forssman antigen, these will be important markers for analyzing cell-surface antigens of mouse teratocarcinoma cells as well as embryos.

References (41)

  • PL Stern et al.

    Monoclonal antibodies as probes for differentiation and tumor-associated antigens: A Forssman specificity on teratocarcinoma stem cells

    Cell

    (1978)
  • PL Stern et al.

    A monoclonal antibody which detects a cell surface antigen on murine embry- onal carcinoma and early mouse embryo stages may recognize a carbohydrate determinant involving α-linked galactose

    J Reprod Immunol

    (1983)
  • LC Stevens

    The development of transplantable teratocarcinomas from intratesticular grafts of pre- and postimplantation mouse embryos

    Dev Biol

    (1970)
  • LM Wiley

    Early embryonic cell-surface antigens as developmental probes

    Curr Top Dev Biol

    (1979)
  • KR Willison et al.

    Expression of a Forssman antigenie specificity in the preimplantation mouse embryo

    Cell

    (1978)
  • T-C Wu et al.

    Immunohistochemical localization of entactin and laminin in mouse embryos and fetuses

    Dev Biol

    (1983)
  • ED Adamson et al.

    Biochemical markers of the progress of differentiation in cloned teratocarcinoma cell lines

    Eur J Biochem

    (1977)
  • K Artzt et al.

    Surface antigens common to mouse cleavage embryos and primitive teratocarcinoma ceils in culture

    Proc Natl Acad Sci USA

    (1973)
  • EG Bernstine et al.

    Alkaline phosphatase activity in mouse teratoma

    Proc Natl Acad Sci USA

    (1973)
  • T Boon et al.

    Tératocarcinome de la souris: Isolement et propriétés de deux lignées de myoblastes

    Ann Microbiol (Paris)

    (1974)
  • Cited by (3)

    View full text