Elsevier

Differentiation

Volume 13, Issue 2, May 1979, Pages 101-108
Differentiation

Classes of Proteins Synthesized in Oocytes, Eggs, Embryos, and Differentiated Tissues of Xenopus Zaevis

https://doi.org/10.1111/j.1432-0436.1979.tb01572.xGet rights and content

Two-dimensional gel electrophoresis has been used to analyse protein synthesis in embryonic stages and in three differentiated tissues of Xenopus laevis. The patterns found in oocytes, unfertilized eggs, embryos shortly after fertilization and at progressively later stages of development have been characterized and compared with the patterns found in the brain, heart and liver of tadpoles. The results suggest that at least four classes of proteins can be recognized among the proteins synthesized, although other categories may exist. They also suggest that some proteins synthesized rapidly in the oocyte are likely to be synthesized in differentiated tissues as well, while proteins synthesized for the first time only after fertilization are much less likely to be synthesized in differentiated tissues.

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      There is a long history of using proteomic approaches to analyze Xenopus oocytes, eggs, and embryos and to identify developmentally relevant proteins. In the early era two-dimensional gel electrophoresis was used to identify proteins that changed in abundance in different samples (Ballantine, Woodland, & Sturgess, 1979; Bravo & Knowland, 1979). The underlying premise was that such changes potentially reflected regulation important for development.

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    Present address: Kemisk Institut, Langelandsgade 140, DK-8000 Aarhus C, Denmark

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