Original Article

Journal of Investigative Dermatology (1971) 56, 311–317; doi:10.1111/1523-1747.ep12261064

TRITIATED THYMIDINE INCORPORATION INTO HAIR FOLLICLE MATRIX AND EPIDERMAL BASAL CELLS AFTER STIMULATION

Christopher S Potten1,2

  1. 1Patterson Laboratories, Christie Hospital, Manchester, M20 9BX, England
  2. 2Cellular and Radiation Biology Laboratories, Allegheny General Hospital, Pittsburgh, Pennsylvania, 15212

Received 17 August 1970; Accepted 27 November 1970.

Top

Abstract

The incorporation of tritiated thymidine into the cells of the epidermis and hair follicle after stimulation of the skin by plucking has been studied. Incorporation levels increase after a time lag of 10 hours. For up to 96 hours the levels show fluctuations which are interpreted as indicating synchronous progression of cells through the cell division cycle. The length of S and the total cycle time for follicle matrix cells and for the basal epidermal cells can be roughly estimated. The values obtained are discussed and compared with those obtained by other techniques.

Top

References

  1. Chase, HB, Montagna, W, Malone, JD: Changes in the skin in relation to the hair growth cycle. Anat. Rec 1953 116: 75,  | Article | PubMed | ISI | ChemPort |
  2. Chase, HB, Rauch, H, Smith, VW: Critical stages of hair development and pigmentation in the mouse . Physiol. Zoo 1951 24: 1,  | ChemPort |
  3. Bullough, WS, Laurence, EB: The mitotic activity of the follicle. pp. 171–188, The Biology of Hair Growth 1958 Eds., Montagna, W. and Ellis, R. A., Academic Press, New York,
  4. Cattaneo, SM, Quastler, H, Sherman, FG: Proliferative cycle in the growing hair follicle of the mouse. Nature 1961 190: 923,  | Article | PubMed | ISI | ChemPort |
  5. Griem, ML: Use of multiple biopsies for the study of the cell cycle of the mouse hair follicle. Nature 1966 210: 213,  | Article | PubMed | ChemPort |
  6. Downs, AM, Chapman, RE, Till, AR, Wilson, PA: Proliferative cycle and fate of cell nuclei in wool follicles. Nature 1966 212: 477,  | Article |
  7. Fry, RJM, Weber, CL, Kisieleski, WE, Griem, ML, Malkinson, FD: Study of cell proliferation of the hair follicle. Proc. IV Int. Cong. Radiat, Res 1970 Gordon & Breach,
  8. Hegazy, MAH, Fowler, JF: Proliferation characteristics of plucked and unplucked mouse skin. Proc. IV Int. Cong. Radiat. Res 1970 Abstract 370,
  9. Moffat, GH, Pele, SR: Delay after plucking of hairs between the appearance of 3H-thymidine in cells and its incorporation into DNA. Exp. Cell Res 1966 42: 460,  | Article | PubMed | ChemPort |
  10. Silver, AF, Chase, HB, Arsenault, CT: Early anagen initiated by plucking compared with early spontaneous anagen. pp 265–286, Advances in Biology of Skin 1969 IX: Ed., Montagna, W., Pergamon Press, New York,
  11. Silver, AF, Chase, HB: DNA synthesis in the adult hair germ during dormancy (telogen) and activation (early aganen). Develop. Biol 1970 21: 440,  | Article | PubMed | ChemPort |
  12. Moffat, GH: The kinetics of cell population in the growing hair follicle of the mouse as revealed by autoradiography using 3H-TdR. Biopathology of Pattern Alopecia 1968 Karger Basel,
  13. Weinstein, GD, Frost, P: Cell proliferation kinetics in benign and malignant skin diseases in human. Nat. Cancer Inst. Mono 1969 30: 225,  | ChemPort |
  14. Devik, F: Studies on the duration of DNA synthesis and mitosis in irradiated and regenerating epidermis cells in mice. Int. J. Radiat. Biol 1962 5: 59,  | PubMed | ISI | ChemPort |
  15. Potten, CS: The x-irradiation of melanocyte precursor cells in resting hair follicles. Proc. VII Int. Pigment Cell Conf Pigmentation: Its Genesis and Biologic Control. Appleton-Century-Crofts, In Press.
  16. Potten, CS: Radiation depigmentation of mouse hair: a study of follicular melanocyte populations. Cell Tissue Kinet 1968 1: 239,  | ChemPort |
  17. Epifanova, OI, Terskikh, VV: On the resting periods in the cell life cycle. Cell Tissue Kinet 1969 2: 75,
  18. Lajtha, LG: Recent studies in erythroid differentiation and proliferation. Medicine (Baltimore) 1964 43: 625,  | PubMed | ChemPort |
  19. Potten, CS, Jessup, BA, Croxson, MB: Incorporation of tritiated thymidine into the skin and hair follicles: II. Daily fluctuations in 3HTdR and 3HUR levels. Cell Tissue Kinet In Press
  20. Potten, CS, Jessup, BA, Croxson, MB: Incorporation of initiated thymidine into the skin and hair follicles: I. Oscillatory changes through the hair growth cycle. Cell Tissue Kinet In Press
  21. Hagemann, RF, Sigdestad, CP, Lesher, S: A method for quantitation of proliferative intestinal mucosal cells on a weight basis: some values for C57B1/6. Cell Tissue Kinet 1970 3: 21,  | PubMed | ChemPort |
  22. Bullough, WS, Laurence, EB: The control of mitotic activity in mouse skin. Dermis and hypodermis. Exp. Cell Res 1960 21: 394,  | Article | ISI |
  23. Benes, L, Rotreklova, E, Fillkuka, J: Decrease in tritium activity in 3H-thymidine labelled cells after Feulgen hydrolysis. Folia Biologica 1969 15: 216,  | PubMed | ChemPort |
  24. Gelfant, S: A new theory on the mechanism of cell division. pp. 229–259, Cell Growth and Cell Division 1963 2: Ed. Harris, R. J. C. Academic Press, New York,

Extra navigation

.
ADVERTISEMENT