Acta Crystallographica Section F

Structural Biology and Crystallization Communications

Volume 63, Part 1 (January 2007)

crystallization communications

Acta Cryst. (2007). F63, 56-58 [ doi:10.1107/S1744309106054509 ]

Purification, crystallization and preliminary crystallographic analysis of the putative thiamine-biosynthesis protein PH1313 from Pyrococcus horikoshii OT3

M. Sugahara, S. Murai, M. Sugahara and N. Kunishima

Abstract: The putative thiamine-biosynthesis protein PH1313 from Pyrococcus horikoshii OT3 has been overexpressed and purified. Crystallization was performed by the oil-microbatch method using 28%(v/v) 2-methyl-2,4-pentanediol as a precipitant at 291 K. A native X-ray diffraction data set at 1.9 Å resolution and a single anomalous dispersion data set from a selenomethionine-derivative crystal at 2.1 Å resolution were collected using synchrotron radiation at 100 K. The native crystal belongs to the orthorhombic space group P2₁2₁2₁, with unit-cell parameters a = 71.7, b = 71.2, c = 141.8 Å.

Keywords: thiamine; 4-thiouridine; ThiI; Pyrococcus horikoshii.