Crystallization and preliminary X-ray analysis of the river buffalo (Bubalus bubalis L.) BB phenotype carbonmonoxyhaemoglobin

Ruminant haemoglobin (Hb) extracted from river buffalo (Bubalus bubalis) has been purified and crystallized. Two different Hb forms of the phenotype BB gave isomorphous crystals which diffracted to 2.8 Å resolution and were not sensitive to radiation damage. Crystals of CO Hb have space group P2₁2₁2₁ with unit-cell parameters a = 54.8, b = 64.0, c = 158.6 Å, and contain one Hb molecule per asymmetric unit.