Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (36)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jander, G.
Right arrow Articles by Last, R. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jander, G.
Right arrow Articles by Last, R. L.
Agricola
Right arrow Articles by Jander, G.
Right arrow Articles by Last, R. L.

Plant Physiol, January 2003, Vol. 131, pp. 139-146

Ethylmethanesulfonate Saturation Mutagenesis in Arabidopsis to Determine Frequency of Herbicide Resistance

Georg Jander,1* Scott R. Baerson,2 Jebecka A. Hudak,3 Kathleen A. Gonzalez, Kenneth J. Gruys, and Robert L. Last4

Cereon Genomics, 45 Sidney Street, Cambridge, Massachusetts 02139 (G.J., J.A.H., R.L.L.); and Monsanto Company, 700 Chesterfield Parkway, St. Louis, Missouri 63198 (S.R.B., K.A.G., K.J.G.)

Plant resistance to glyphosate has been reported far less frequently than resistance to sulfonylurea and imidazolinone herbicides. However, these studies tend to be anecdotal, without side by side comparisons for a single species or natural isolate. In this study, we tested the frequencies of resistance of three herbicides in a controlled ethylmethanesulfonate (EMS) saturation mutagenesis experiment, allowing a direct comparison of the frequencies at which resistant mutant plants arise. The 100% growth inhibition dose rates of glyphosate, chlorsulfuron (a sulfonylurea herbicide), and imazethapyr (an imidazolinone herbicide) were determined for Arabidopsis. Populations of EMS-mutagenized M2 seedlings were sprayed with twice the 100% growth inhibition dose of glyphosate, chlorsulfuron, or imazethapyr, and herbicide-resistant mutants were identified. Although there were no glyphosate-resistant mutants among M2 progeny of 125,000 Columbia and 125,000 Landsberg erecta M1 lines, chlorsulfuron resistance and imazethapyr resistance each appeared at frequencies of 3.2 × 10-5. Given the observed frequency of herbicide resistance mutations, we calculate that there are at least 700 mutations in each EMS-mutagenized Arabidopsis line and that fewer than 50,000 M1 lines are needed to have a 95% chance of finding a mutation in any given G:C base pair in the genome. As part of this study, two previously unreported Arabidopsis mutations conferring resistance to imidazolinone herbicides, csr1-5 (Ala-122-Thr) and csr1-6 (Ala-205-Val), were discovered. Neither of these mutations caused enhanced resistance to chlorsulfuron in Arabidopsis.

1 Present address: Boyce Thompson Institute for Plant Research, Tower Road, Ithaca, NY 14853.

2 Present address: U.S. Department of Agriculture, Agricultural Research Service, Natural Products Utilization Research Unit, P.O. Box 8048, University, MS 38677.

3 Present address: Infinity Pharmaceuticals, 650 Albany Street, Boston, MA 02118.

4 Present address: Max Planck Institut for Chemical Ecology, Beutenberg Campus, Winzerlaer Strasse 10, 07745, Jena, Germany.

* Corresponding author; e-mail gj32{at}cornell.edu; fax 607-254-2958.

© 2003 American Society of Plant Biologists


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
Y. Lu, L. J. Savage, I. Ajjawi, K. M. Imre, D. W. Yoder, C. Benning, D. DellaPenna, J. B. Ohlrogge, K. W. Osteryoung, A. P. Weber, et al.
New Connections across Pathways and Cellular Processes: Industrialized Mutant Screening Reveals Novel Associations between Diverse Phenotypes in Arabidopsis
Plant Physiology, April 1, 2008; 146(4): 1482 - 1500.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
Y. Manabe, N. Tinker, A. Colville, and B. Miki
CSR1, the Sole Target of Imidazolinone Herbicide in Arabidopsis thaliana
Plant Cell Physiol., September 1, 2007; 48(9): 1340 - 1358.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. Gressel and A. A. Levy
Agriculture: The selector of improbable mutations
PNAS, August 15, 2006; 103(33): 12215 - 12216.
[Full Text] [PDF]

Home page
 GeneticsHome page
F. Roux, C. Camilleri, S. Giancola, D. Brunel, and X. Reboud
Epistatic Interactions Among Herbicide Resistances in Arabidopsis thaliana: The Fitness Cost of Multiresistance
Genetics, November 1, 2005; 171(3): 1277 - 1288.
[Abstract] [Full Text] [PDF]

Home page
 Crop Sci.Home page
C. J. Pozniak, I. T. Birk, L. S. O'Donoughue, C. Menard, P. J. Hucl, and B. K. Singh
Physiological and Molecular Characterization of Mutation-Derived Imidazolinone Resistance in Spring Wheat
Crop Sci., July 1, 2004; 44(4): 1434 - 1443.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
F. Roux, J. Gasquez, and X. Reboud
The Dominance of the Herbicide Resistance Cost in Several Arabidopsis thaliana Mutant Lines
Genetics, January 1, 2004; 166(1): 449 - 460.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
E. A. Greene, C. A. Codomo, N. E. Taylor, J. G. Henikoff, B. J. Till, S. H. Reynolds, L. C. Enns, C. Burtner, J. E. Johnson, A. R. Odden, et al.
Spectrum of Chemically Induced Mutations From a Large-Scale Reverse-Genetic Screen in Arabidopsis
Genetics, June 1, 2003; 164(2): 731 - 740.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 2003 by the American Society of Plant Biologists