First published online August 16, 2002; 10.1104/pp.005041
Plant Physiol, September 2002, Vol. 130, pp. 234-243
Arabidopsis UVR8 Regulates Ultraviolet-B Signal
Transduction and Tolerance and Contains Sequence Similarity to
Human Regulator of Chromatin Condensation 1
Daniel J.
Kliebenstein,1*
Jackie E.
Lim,2
Laurie G.
Landry,3 and
Robert L.
Last4
The Boyce Thompson Institute for Plant Research and Section of
Genetics and Development, Cornell University, Ithaca, New York
14853
To further our understanding of how plants defend against the
harmful effects of ultraviolet (UV) light, we characterized an
Arabidopsis mutant hypersensitive to UV-B. This mutant, UV resistance locus 8-1 (uvr8-1), contains a single
recessive mutation at the bottom of chromosome 5. Fine-scale mapping
localized uvr8-1 to a 21-kb locus containing five
predicted open reading frames. Sequencing of this entire region
revealed that the uvr8-1 allele contains a 15-nucleotide
deletion in a gene similar to the human guanine nucleotide exchange
factor regulator of chromatin condensation 1. This mutation reduces the
UV-B-mediated induction of flavonoids and blocks chalcone synthase mRNA
and protein induction. In contrast, uvr8-1 has enhanced
induction of PR1 and PR5 proteins in response to UV-B, an indication of
increased UV-B injury. These results suggest that UVR8
acts in a UV-B signal transduction pathway leading to induction of
flavonoid biosynthesis.
1
Present address: Department of Vegetable Crops,
University of California, One Shields Avenue, Davis, CA 95616.
2
Present address: Department of Genetics, Division of
Medical Sciences, Harvard Medical School, Boston, MA 02215.
3
Present address: Department of Plant Breeding, Cornell
University, Ithaca, NY 14853.
4
Present address: Cereon Genomics LLC, 45 Sidney Street,
Cambridge, MA 02139.
*
Corresponding author; e-mail Kliebenstein{at}ucdavis.edu; fax
530-752-9659.
© 2002 American Society of Plant Physiologists
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