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PLANT PHYSIOLOGY , Vol 113, Issue 4 1437-1445, Copyright © 1997 by American Society of Plant Biologists
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GENE REGULATION AND MOLECULAR GENETICS |
Characterization of Flavonol Synthase and Leucoanthocyanidin Dioxygenase Genes in Arabidopsis (Further Evidence for Differential Regulation of "Early" and "Late" Genes)
M. K. Pelletier, J. R. Murrell and B. W. Shirley
Department of Biology, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061-0406
As part of an ongoing investigation into the organization and regulation of
the flavonoid biosynthetic pathway, two Arabidopsis thaliana expressed
sequence tag (EST) clones (153O10T7 and YAY780) with high homology to
leucoanthocyanidin dioxygenase (LDOX) or flavonol synthase (FLS) were
identified. EST YAY780 was sequenced and found to encode a protein 49 to
78% identical to all LDOX sequences in the database. EST 153O10T7 was used
to isolate a genomic clone encoding a protein with 59 to 61% sequence
identity to petunia (Petunia hybrida) and potato (Solanum tuberosum) FLS.
DNA blot analysis was used to screen the Arabidopsis genome for sequences
related to FLS and LDOX and to determine the positions of the two clones on
the RI map. The expression patterns of FLS and LDOX in etiolated seedlings
moved to white light and in two putative regulatory mutants (ttg and tt8)
were determined by RNA blot analysis. These studies indicate that FLS is an
"early" flavonoid gene in Arabidopsis seedlings, whereas LDOX is a "late"
gene. Furthermore, FLS is the first flavonoid enzyme identified in
Arabidopsis that may be encoded by a gene family.
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