An In Vitro Assay for the Specific Induction and Regression of Puffs in Isolated Polytene Nuclei of Drosophila melanogaster
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Excerpt
Drosophila melanogaster has several characteristics favorable for studying the control of gene expression in eukaryotes. One of the greatest potential advantages is the reservoir of genetic information and the consequent sophistication of genetic techniques which may be employed. Genetic studies, in concert with other techniques, will provide the ultimate proof of control of expression. A second feature is the presence of highly polytene nuclei in some larval tissues. Autoradiographic techniques have exploited the cytology as a means of determining which regions of the genome are transcriptionally active and, coupled with hybridization, of determining from what regions a purified RNA was derived.
Recent work on gene expression in Drosophila has concentrated on two sets of genes which can be experimentally induced in excised salivary glands. The puffing pattern, originally described by Ritossa (1962) as a response to elevated temperature or exposure to dinitrophenol (DNP), can also be reproduced in isolated glands...
