Studies on the Transfer RNA Genes of Drosophila
This extract was created in the absence of an abstract.
Excerpt
The in situ hybridization technique developed by Gall and Pardue (1969) offers great potential for the analysis of chromosome structure by the direct localization of genes and specific DNA sequences. However, this method relies on the availability of a purified RNA or single-stranded DNA radioactively labeled to a specific activity high enough to produce sufficient grains at the site of hybridization. The minimal required specific activity is largely dependent on the number of binding sites on the chromosome available to the incoming RNA or DNA.
The successful use of the in situ hybridization technique has been in the localization of highly repetitive DNA sequences in diploid cells (Pardue and Gall, 1970; Jones, 1970; Pardue et al., 1970; Lambert et al., 1972; Henderson et al., 1972), the localization of highly amplified ribosomal genes in oocytes of the toad, Xenopus, (Gall and Pardue, 1969; Pardue and Gall, 1970), and the localization of...
