Studies on the Primary Structure and the Replication Mechanism of Bacteriophage RNA
This extract was created in the absence of an abstract.
Excerpt
The RNA of the bacteriophage MS2 carries the genetic information for the synthesis of at least three viral proteins. Furthermore it is specifically recognized and replicated via a complementary strand by a viral RNA polymerase (Haruna, Nozu, Ohtaka, and Spiegelman, 1963; Fiers, Verplancke, and Van Styvendaele, 1967). In addition, it forms a complex with the homologous coat protein (Sugiyama, Hebert, and Hartman, 1967; Hohn, 1969). These processes involve highly specific interactions, which can only be understood in detail if more insight into the structure of the viral RNA is achieved.
THE PRIMARY STRUCTURE OF VIRAL RNA
The Terminal Sequences of MS2 RNA
Sugiyama (1965) has shown that the 3′-end of MS2 RNA is an unsubstituted adenosine residue, preceded by a pyrimidine. We have specifically labeled this 3′OH-end by periodate oxidation followed by reduction with tritiated sodium boro-hydride (RajBhandary et al., 1966). In this way it was possible to detect and...
