Peptide Chain Termination, Codon, Protein Factor, and Ribosomal Requirements
This extract was created in the absence of an abstract.
Excerpt
Results of biochemical studies (Last et al., 1967; Capecchi, 1967; Bretscher, 1968) and genetic studies (Weigert and Garen, 1965; Brenner, Stretton, and Kaplan, 1965) show that the synthesis of a peptide chain is terminated upon translation of messenger RNA codons UAA or UAG. Genetic evidence also indicates UGA functions as a barrier or terminator codon (Brenner, Barnett, Katz, and Crick, 1967; Weigert, Lanka, and Garen, 1967).
The mechanism of peptide chain termination has been investigated by stimulating cell-free protein synthesis with randomly ordered or sequenced polyribonucleotides (Takanami and Y. H. Yan, 1965; Bretscher et al., 1965; Ganoza and Nakamoto, 1966; Morgan, Wells, and Khorana, 1966; Kössel, 1968), or viral RNA (Capecchi, 1967; Bretscher, 1968). Translation of UAA or UAG results in the release of free peptides from ribosomes. Capecchi (1967) partially purified a factor required for the release of nascent peptide chains from ribosomes upon the translation of the amber...
