Topic Introduction

Culturing Mouse Tumor Cells

  1. Anil K. Rustgi3,4
  1. 1Division of Gastroenterology, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan 48109;
  2. 2European Molecular Biology Laboratory, Mouse Biology Unit, 00015 Monterotondo, Italy;
  3. 3Gastroenterology Division, Department of Medicine, Department of Genetics and Abramson Cancer Center, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104

    Abstract

    Genetically engineered mouse models (GEMMs) are attractive for the study of cancer; however, they can be time-consuming and expensive to produce and maintain. Thus, in certain contexts, the use of in vitro culture systems of tumor cells may provide an efficient and effective means to test hypotheses before assessment in or to complement discoveries in GEMMs. This introduction will briefly review the issues pertaining to in vitro analyses of primary cancer cells and highlight several “best practice” protocols that can be used when working with diverse types of carcinomas.

    Footnotes

    • ↵4 Correspondence: anil2{at}mail.med.upenn.edu

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    1. doi:10.1101/pdb.top069989 Cold Spring Harb Protoc 2015: pdb.top069989- © 2015 Cold Spring Harbor Laboratory Press
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