Two-Step Bacterial Artificial Chromosome (BAC) Engineering: Electroporation of Competent BAC Host Cells with the Recombinant Shuttle Vector
Abstract
Bacterial artificial chromosome (BAC) clones are rendered electrocompetent and transformed with the recombinant shuttle vector, pLD53SCAB/AB-box. Cointegrates are selected by growth on chloramphenicol and ampicillin to ensure recombination of the shuttle vector into the BAC.
Footnotes
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From the Molecular Cloning collection, edited by Michael R. Green and Joseph Sambrook.
