Protocol

Calcium Phosphate-Mediated Transfection of Eukaryotic Cells with Plasmid DNAs

  1. Pradeep D. Uchil

Abstract

This protocol describes a calcium phosphate-mediated transfection method for use with plasmid DNAs and adherent cells. At the end of the protocol is an alternative method for high-efficiency generation of stable transfectants.

Footnotes

  • From the Molecular Cloning collection, edited by Michael R. Green and Joseph Sambrook.

| Table of Contents

This Article

  1. doi:10.1101/pdb.prot095430 Cold Spring Harb Protoc 2019: pdb.prot095430- © 2019 Cold Spring Harbor Laboratory Press
  1. » Abstract
  2. Full Text
  3. Full Text (PDF)

Article Category

  1. Protocol

Personal Folder

  1. Save to Personal Folders

Updates/Comments

  1. Submit Updates/Comments
  2. No Updates/Comments published
  3. Alert me when Updates/Comments are published

Share

Current Issue

  1. May 2024, 2024 (5)
  1. Current Issue