Corrigendum: Methods to Synthesize Large DNA Fragments for a Synthetic Yeast Genome
- Yizhi Cai and
- Junbiao Dai
Cold Spring Harb Protoc 2017; doi: 10.1101/pdb.prot080978
When this article was first published, the reagent PEG-8000 was incorrectly listed as “PEG-800” in the recipe entitled “ISO Buffer (5×).” The authors apologize for this error. A corrected version of the recipe is below. In addition, the HTML version of the recipe (doi:10.1101/pdb.rec090001) and the PDF version of the article have been amended accordingly.
ISO Buffer (5×)
| 1 m Tris–HCl (pH 7.5) (Invitrogen 15567-027) | 3000 µL |
| 1 m MgCl2 (Sigma-Aldrich M1028) | 300 µL |
| 100 mm dGTP | 60 µL |
| 100 mm dTTP | 60 µL |
| 100 mm dATP | 60 µL |
| 100 mm dCTP | 60 µL |
| 1 m DTT (Sigma-Aldrich D9779) | 300 µL |
| PEG-8000 (USB Affymetrix 19959) | 1.5 g |
| 100 mm NAD (Sigma-Aldrich N1511) | 300 µL |
| H2O | to 6 mL |
| Combine the above ingredients. Store 40-µL aliquots at −20°C. | |
