Chromosomal localization and expressed sequence tag generation of clones from a normalized human adult thymus cDNA library.

  1. J E Lamerdin,
  2. R S Athwal,
  3. M S Kansara,
  4. A K Sandhu,
  5. S R Patanjali,
  6. S M Weissman, and
  7. A V Carrano
  1. Human Genome Center, Lawrence Livermore National Laboratory, Livermore, California 94550, USA. lamerdin@llnl.gov

Abstract

Expressed sequence tags (ESTs) from 298 clones have been generated from a randomly primed, normalized human adult thymus cDNA library. We describe the chromosomal localization of 136 of these ESTs by PCR-based mapping to a human monochromosomal somatic cell hybrid panel. Data base similarities to known genes are also described. A subset (n = 18) of these randomly primed ESTs extended the sequence of ESTs from other tissues currently in dbEST. Of the nonrepetitive human adult thymus ESTs generated in this study, 237 (79.5%) have no similarity to current data base entries. This would suggest that our collection contains approximately 100 new coding regions from thymus tissue, a large proportion of which likely will represent the middle regions of genes. The mapped ESTs should prove useful as new gene-based markers for mapping and candidate gene hunting, particularly when anchored to a well-developed physical map of the human genome.

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  1. doi: 10.1101/gr.5.4.359 Genome Res. 5: 359-367 Copyright © Cold Spring Harbor Laboratory Press

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