Human chromosome-specific cDNA libraries: new tools for gene identification and genome annotation.

  1. R G Del Mastro,
  2. L Wang,
  3. A D Simmons,
  4. T D Gallardo,
  5. G A Clines,
  6. J A Ashley,
  7. C J Hilliard,
  8. J J Wasmuth,
  9. J D McPherson, and
  10. M Lovett
  1. Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas 75235-8591, USA.

Abstract

To date, only a small percentage of human genes have been cloned and mapped. To facilitate more rapid gene mapping and disease gene isolation, chromosome 5-specific cDNA libraries have been constructed from five sources. DNA sequencing and regional mapping of 205 unique cDNAs indicates that 25 are from known chromosome 5 genes and 138 are from new chromosome 5 genes (a frequency of 79.5%). Sequence complexity estimates indicate that each library contains -20% of the approximately 5000 genes that are believed to reside on chromosome 5. This study more than doubles the number of genes mapped to chromosome 5 and describes an important new tool for disease gene isolation.

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  1. doi: 10.1101/gr.5.2.185 Genome Res. 5: 185-194 Copyright © Cold Spring Harbor Laboratory Press

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