A sensitive, quantitative assay for measurement of allele-specific transcripts differing by a single nucleotide.

  1. J Singer-Sam,
  2. J M LeBon,
  3. A Dai and
  4. A D Riggs
  1. Biology Division, Beckman Research Institute, City of Hope, Duarte, CA 91010.

Abstract

We have found that the single nucleotide primer extension assay, a PCR-based assay currently used qualitatively to measure allelic differences in DNA, can be used quantitatively to measure allele-specific transcripts differing by only a single nucleotide. We show that total RNA containing the Pgk-1a transcript can be specifically detected even in a 1000-fold excess of RNA containing the Pgk-1b transcript.

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  1. doi: 10.1101/gr.1.3.160 Genome Res. 1: 160-163 Copyright © Cold Spring Harbor Laboratory Press

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