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RESEARCH PAPER
1 Department of Molecular and Cell Biology, 2 Center for Integrative Genomics, 3 Plant and Microbial Biology, University of California, Berkeley, California 94720-3204 USA
To gain insight into splicing regulation, we developed a microarray to assay all annotated alternative splicing events in Drosophila melanogaster and identified the alternative splice events controlled by four splicing regulators: dASF/SF2, B52/SRp55, hrp48, and PSI. The number of events controlled by each of these factors was found to be highly variable: dASF/SF2 strongly affects >300 splicing events, whereas PSI strongly affects only 43 events. Pairwise analysis also revealed many instances of splice site usage affected by multiple factors and provides the framework to understand the network controlling the alternatively spliced mRNA isoforms that compose the Drosophila transcriptome.
[Keywords: Alternative splicing; SR proteins; hnRNP proteins; microarray; Drosophila melanogaster]
Received March 10, 2005; revised version accepted April 20, 2005.
Article and publication are at http://www.genesdev.org/cgi/doi/10.1101/gad.1314205.
4 These authors contributed equally to this work
5 Corresponding author.
E-MAIL don_rio{at}berkeley.edu; FAX (510) 642-6062.
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