Homologous Recombination in Mouse L Cells
- F.-L. Lin,
- K. Sperle, and
- N. Sternberg
This extract was created in the absence of an abstract.
Excerpt
Our present understanding of how cells recombine homologous DNA sequences sterns from many elegant studies in bacteria and yeast (Radding 1982; Szostak et al. 1983). These studies owe much of their success to the availability of chromosomal and viral markers that can be used to follow recombination, to the existence of mutations in genes that encode recombinase proteins, to the increasing ability of scientists to manipulate recombination substrates, and to the existence of in vitro systems that mimic the in vivo reactions. None of these capabilities is presently available for studying homologous recombination in mammalian somatic cells. Traditionally, the latter studies have employed viruses as convenient substrates and have shown that homologous recombination can be detected (Ginsburg and Young 1977; DasGupta and Summers 1980; Vogel 1980; Young and Silverstein 1980), that it is independent of viral replication (Dubbs et al. 1974), and that it may involve intermediates, such as “Holliday”...
