Abstract
Antibody neutralization is an important prognostic factor in many viral diseases. To easily and rapidly measure titers of neutralizing antibodies in serum or plasma, we developed pseudovirion particles composed of the spike glycoprotein of SARS-CoV-2 incorporated onto murine leukemia virus capsids and a modified minimal MLV genome encoding firefly luciferase. These pseudovirions provide a practical means of assessing immune responses under laboratory conditions consistent with biocontainment level 2.
Competing Interest Statement
The authors have declared no competing interest.
Footnotes
We have added plasma samples from additional participants.
Abbreviations
- SARS
- severe acute respiratory syndrome
- SARS-CoV-2
- severe acute respiratory syndrome coronavirus 2
- COVID-19
- coronavirus disease 2019
- DMEM
- Dulbecco’s modified Eagle’s medium
- FBS
- fetal bovine serum
- RBD
- receptor binding domain
- NT
- neutralizing titer
- MLV
- murine leukemia virus
- VSV-G
- vesicular stomatitis virus glycoprotein
- HIV
- human immunodeficiency virus
- ACE2
- Angiotensin Converting Enzyme 2
Copyright
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.