Large-scale gene trapping in C57BL/6N mouse embryonic stem cells
- Gwenn M. Hansen1,3,4,
- Diane C. Markesich1,3,
- Michael B. Burnett1,
- Qichao Zhu1,
- Karen M. Dionne1,
- Lizabeth J. Richter1,
- Richard H. Finnell2,
- Arthur T. Sands1,
- Brian P. Zambrowicz1, and
- Alejandro Abuin1
- 1 Lexicon Pharmaceuticals Incorporated, The Woodlands, Texas 77381, USA;
- 2 Texas A&M Institute for Genomic Medicine, Houston, Texas 77030, USA
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↵3 These authors contributed equally to this work.
Abstract
We report the construction and analysis of a mouse gene trap mutant resource created in the C57BL/6N genetic background containing more than 350,000 sequence-tagged embryonic stem (ES) cell clones. We also demonstrate the ability of these ES cell clones to contribute to the germline and produce knockout mice. Each mutant clone is identified by a genomic sequence tag representing the exact insertion location, allowing accurate prediction of mutagenicity and enabling direct genotyping of mutant alleles. Mutations have been identified in more than 10,000 genes and show a bias toward the first intron. The trapped ES cell lines, which can be requested from the Texas A&M Institute for Genomic Medicine, are readily available to the scientific community.
Footnotes
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↵4 Corresponding author.
↵4 E-mail ghansen{at}lexpharma.com; fax (281) 863-8088.
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[Supplemental material is available online at www.genome.org.]
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Article published online before print. Article and publication are at http://www.genome.org/cgi/doi/10.1101/gr.078352.108.
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- Received March 13, 2008.
- Accepted July 21, 2008.
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Freely available online through the Genome Research Open Access option.
- Copyright © 2008, Cold Spring Harbor Laboratory Press
