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Volume 156, Issue 4

Glucose and glucose 6-phosphate as carbon sources in extra- and intracellular growth of enteroinvasive and Free

Abstract

To study the role of carbohydrates, in particular glucose, glucose 6-phosphate and mannose, as carbon substrates for extra- and intracellular replication of facultative intracellular enteric bacteria, mutants of two enteroinvasive (EIEC) strains and a serovar Typhimurium isolate were constructed that were defective in the uptake of glucose and mannose (Δ, ), glucose 6-phosphate (Δ) or all three carbohydrates (Δ, , ). The ability of these mutants to grow in RPMI medium containing the respective carbohydrates and in Caco-2 cells was compared with that of the corresponding wild-type strains. In the three strains, deletions of , or resulted in considerably different levels of inhibition of growth in the presence of glucose, mannose and glucose 6-phosphate, respectively, but hardly reduced their capability for intracellular replication in Caco-2 cells. Even the triple mutants Δ, , of the three enterobacterial strains were still able to replicate in Caco-2 cells, albeit at strain-specific lower rates than the corresponding wild-type strains.

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Keyword(s): CCR, carbon catabolite repression , EIEC, enteroinvasive Escherichia coli , FCS, fetal calf serum , Glc, glucose , Glc6P, glucose 6-phosphate , LB, Luria–Bertani , Man, mannose , p.i., post-infection , PEP, phosphoenolpy-ruvate , PTS, phosphoenolpyruvate : , carbohydrate phosphotransferase system , SCV, Salmonella-containing vacuole and TSB, tryptic soy broth
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2010-04-01
2024-04-16
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Glucose and glucose 6-phosphate as carbon sources in extra- and intracellular growth of enteroinvasive Escherichia coli and Salmonella enterica
Microbiology 156, 1176 (2010); https://doi.org/10.1099/mic.0.034744-0
/content/journal/micro/10.1099/mic.0.034744-0
/content/journal/micro/10.1099/mic.0.034744-0
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