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Volume 9, Issue 10

Contrasting genes conferring short- and long-term biofilm adaptation in Open Access

Abstract

is an opportunistic food-borne bacterium that is capable of infecting humans with high rates of hospitalization and mortality. Natural populations are genotypically and phenotypically variable, with some lineages being responsible for most human infections. The success of is linked to its capacity to persist on food and in the environment. Biofilms are an important feature that allow these bacteria to persist and infect humans, so understanding the genetic basis of biofilm formation is key to understanding transmission. We sought to investigate the biofilm-forming ability of by identifying genetic variation that underlies biofilm formation in natural populations using genome-wide association studies (GWAS). Changes in gene expression of specific strains during biofilm formation were then investigated using RNA sequencing (RNA-seq). Genetic variation associated with enhanced biofilm formation was identified in 273 genes by GWAS and differential expression in 220 genes by RNA-seq. Statistical analyses show that the number of overlapping genes flagged by either type of experiment is less than expected by random sampling. This novel finding is consistent with an evolutionary scenario where rapid adaptation is driven by variation in gene expression of pioneer genes, and this is followed by slower adaptation driven by nucleotide changes within the core genome.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): biofilm , genome-wide association study , genomics , Listeria and RNAseq
Funding
This study was supported by the:
  • Medical Research Council (Award MR/L015080/1)
    • Principle Award Recipient: SamuelK Sheppard
  • Medical Research Council (Award MR/S009264/1)
    • Principle Award Recipient: SamuelK Sheppard
  • Medical Research Council (Award MR/V001213/1)
    • Principle Award Recipient: SamuelK Sheppard
© 2023 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2023-10-18
2024-05-21
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Contrasting genes conferring short- and long-term biofilm adaptation in Listeria
M Gen 9, 001114 (2023); https://doi.org/10.1099/mgen.0.001114
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