1887

Abstract

The aim of this study was to characterize the resistance mechanism in four clinical and five intestinal vancomycin-resistant strains with VanB phenotype recovered from unrelated patients confined in two Spanish hospitals and to determine their clonal relationships. MIC values for vancomycin and teicoplanin were 16–32 and 0.5 μg ml, respectively. The mechanism of vancomycin resistance, as well as the genetic environment of the implicated gene, was analysed by PCR and sequencing. The gene was detected in all nine strains and the intergenic region showed the characteristic mutations of the subtype. Two possibly related PFGE patterns, A (seven strains) and B (two strains), were distinguished among these enterococci. The intergenic region was amplified in the nine strains and two amino acid changes were detected in the protein encoded by the gene in strains of pattern A with respect to those of pattern B. The gene cluster was integrated into Tn in all nine strains, being gene-linked to this transposon. The (6′)-, (3′)- and (B) genes were also detected in all of the strains. Both isolates with PFGE pattern B contained the gene. In summary, -containing strains with indistinguishable PFGE patterns were recovered from seven patients from two Spanish hospitals.

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2006-09-01
2024-03-29
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