1887

Abstract

Atypical porcine pestivirus (APPV) causes congenital tremor (CT) in piglets and has a wide geographical distribution. In this study, we evaluated APPV prevalence using 165 piglet sera from southwest China. Viral RNA was detectable by qRT-PCR in 43.6 % (17/39, 95 % CI 27.8–60.4 %) of piglets with CT, while viral RNA was not detected in the sera of any healthy piglets. The seven complete APPV genomes were obtained from distinct farms and were 11 269–11 459 nucleotides in length. The genomes of the seven strains shared 82.8–98 % identity with the APPV reference strains. Phylogenetic analysis of the complete genomes as well as E2 and Nrpo sequences revealed that the seven APPVs clustered into two groups: four strains belonged to genogroups A and D and three strains belonged to a novel APPV genotype, tentatively called genogroup E. This study provides important insights into the epidemiological features and genetic diversity of APPV.

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2018-12-05
2024-04-18
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References

  1. Postel A, Hansmann F, Baechlein C, Fischer N, Alawi M et al. Presence of atypical porcine pestivirus (APPV) genomes in newborn piglets correlates with congenital tremor. Sci Rep 2016; 6:27735 [View Article][PubMed]
    [Google Scholar]
  2. Smith DB, Meyers G, Bukh J, Gould EA, Monath T et al. Proposed revision to the taxonomy of the genus Pestivirus, family Flaviviridae. J Gen Virol 2017; 98:2106–2112 [View Article][PubMed]
    [Google Scholar]
  3. Hause BM, Collin EA, Peddireddi L, Yuan F, Chen Z et al. Discovery of a novel putative atypical porcine pestivirus in pigs in the USA. J Gen Virol 2015; 96:2994–2998 [View Article][PubMed]
    [Google Scholar]
  4. de Groof A, Deijs M, Guelen L, van Grinsven L, van Os-Galdos L et al. Atypical Porcine Pestivirus: A Possible Cause of Congenital Tremor Type A-II in Newborn Piglets. Viruses 2016; 8:E271 [View Article][PubMed]
    [Google Scholar]
  5. Arruda BL, Arruda PH, Magstadt DR, Schwartz KJ, Dohlman T et al. Identification of a divergent lineage porcine pestivirus in nursing piglets with congenital tremors and reproduction of disease following experimental inoculation. PLoS One 2016; 11:e0150104 [View Article][PubMed]
    [Google Scholar]
  6. Beer M, Wernike K, Dräger C, Höper D, Pohlmann A et al. High prevalence of highly variable atypical porcine pestiviruses found in Germany. Transbound Emerg Dis 2017; 64:e22e26 [View Article][PubMed]
    [Google Scholar]
  7. Yuan J, Han Z, Li J, Huang Y, Yang J et al. Atypical porcine pestivirus as a novel type of pestivirus in pigs in China. Front Microbiol 2017; 8:862 [View Article][PubMed]
    [Google Scholar]
  8. Gatto IRH, Harmon K, Bradner L, Silva P, Linhares DCL et al. Detection of atypical porcine pestivirus in Brazil in the central nervous system of suckling piglets with congenital tremor. Transbound Emerg Dis 2018; 65:375–380 [View Article][PubMed]
    [Google Scholar]
  9. Postel A, Meyer D, Cagatay GN, Feliziani F, de Mia GM et al. High abundance and genetic variability of atypical porcine pestivirus in pigs from Europe and Asia. Emerg Infect Dis 2017; 23:2104–2107 [View Article][PubMed]
    [Google Scholar]
  10. Gatto IRH, Arruda PH, Visek CA, Victoria JG, Patterson AR et al. Detection of atypical porcine pestivirus in semen from commercial boar studs in the United States. Transbound Emerg Dis 2018; 65:e339e343 [View Article][PubMed]
    [Google Scholar]
  11. Yeşilbağ K, Alpay G, Becher P. Variability and global distribution of subgenotypes of bovine viral diarrhea virus. Viruses 2017; 9:E128 [View Article][PubMed]
    [Google Scholar]
  12. Weber MN, Streck AF, Silveira S, Mósena ACS, da Silva MS et al. Homologous recombination in pestiviruses: identification of three putative novel events between different subtypes/genogroups. Infect Genet Evol 2015; 30:219–224 [View Article][PubMed]
    [Google Scholar]
  13. Greiser-Wilke I, Depner K, Fritzemeier J, Haas L, Moennig V. Application of a computer program for genetic typing of classical swine fever virus isolates from Germany. J Virol Methods 1998; 75:141–150 [View Article][PubMed]
    [Google Scholar]
  14. Zhang H, Wen W, Hao G, Hu Y, Chen H et al. Phylogenetic and genomic characterization of a novel atypical porcine pestivirus in China. Transbound Emerg Dis 2018; 65:e202e204 [View Article][PubMed]
    [Google Scholar]
  15. Silveira S, Weber MN, Mósena AC, da Silva MS, Streck AF et al. Genetic Diversity of Brazilian Bovine Pestiviruses Detected Between 1995 and 2014. Transbound Emerg Dis 2017; 64:613–623 [View Article][PubMed]
    [Google Scholar]
  16. Zhang K, Wu K, Liu J, Ge S, Xiao Y et al. Identification of atypical porcine pestivirus infection in swine herds in China. Transbound Emerg Dis 2017; 64:1020–1023 [View Article][PubMed]
    [Google Scholar]
  17. Schwarz L, Riedel C, Högler S, Sinn LJ, Voglmayr T et al. Congenital infection with atypical porcine pestivirus (APPV) is associated with disease and viral persistence. Vet Res 2017; 48:1 [View Article][PubMed]
    [Google Scholar]
  18. Ni W, Hu S, Qiao J, Yu Y, Wang D et al. Suppression of bovine viral diarrhea virus replication by single and dual short hairpin RNA-mediated RNA interference. Res Vet Sci 2012; 93:544–548 [View Article][PubMed]
    [Google Scholar]
  19. Rios L, Coronado L, Naranjo-Feliciano D, Martínez-Pérez O, Perera CL et al. Deciphering the emergence, genetic diversity and evolution of classical swine fever virus. Sci Rep 2017; 7:17887 [View Article][PubMed]
    [Google Scholar]
  20. Hamers C, Dehan P, Couvreur B, Letellier C, Kerkhofs P et al. Diversity among bovine pestiviruses. Vet J 2001; 161:112–122 [View Article][PubMed]
    [Google Scholar]
  21. Kolaskar AS, Tongaonkar PC. A semi-empirical method for prediction of antigenic determinants on protein antigens. FEBS Lett 1990; 276:172–174 [View Article][PubMed]
    [Google Scholar]
  22. Hossain R, Yasmin T, Hosen MI, Nabi A. In silico identification of potential epitopes present in human adenovirus proteins for vaccine design and of putative drugs for treatment against viral infection. J Immunol Methods 2018; 455:55–70 [View Article][PubMed]
    [Google Scholar]
  23. Couto J, Antunes S, Ferrolho J, de La Fuente J, Domingos A. Reduction of Mosquito survival in mice vaccinated with Anopheles stephensi glucose transporter. Biomed Res Int 2017; 2017:1–8 [View Article][PubMed]
    [Google Scholar]
  24. Becher P, Tautz N. RNA recombination in pestiviruses: cellular RNA sequences in viral genomes highlight the role of host factors for viral persistence and lethal disease. RNA Biol 2011; 8:216–224 [View Article][PubMed]
    [Google Scholar]
  25. He CQ, Ding NZ, Chen JG, Li YL. Evidence of natural recombination in classical swine fever virus. Virus Res 2007; 126:179–185 [View Article][PubMed]
    [Google Scholar]
  26. Martin DP, Murrell B, Golden M, Khoosal A, Muhire B. RDP4: Detection and analysis of recombination patterns in virus genomes. Virus Evol 2015; 1:vev003 [View Article][PubMed]
    [Google Scholar]
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