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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 7

sp. nov., from phycosphere microbiota of toxic marine dinoflagellate Free

Abstract

A taxonomic study was carried out on a novel algae-associated bacterial strain Z3-1, which was isolated from phycosphere microbiota of toxic marine dinoflagellate 880. Cells of strain Z3-1 were Gram-stain-negative, rod-shaped and strictly aerobic and were motile by means of flagella. Strain Z3-1 grew at 25–42 °C, pH 5.0–10.0 and 1.0–5.0 % (w/v) NaCl. Strain Z3-1 reduced nitrate to nitrite, but did not reduce nitrite to nitrogen gas. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain Z3-1 belongs to the genus showing the highest sequence similarity (97.0 %) to JCM 14935. The average nucleotide identity and digital DNA–DNA hybridization relatedness between strain Z3-1 and type strains of genus with available genome sequences were in the ranges of 72.4–74.4 % and 22.7–23.3 %, respectively. The major fatty acids were summed in feature 8 (C 7c and/or C 6c), C 8c cyclo, C 7c 11-metyl and iso-C. The major polar lipids were determined as diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, two unidentified phospholipids and four unidentified polar lipids. The genomic DNA G+C content calculated from genome sequence was 65.6 mol%. Based on genotypic, chemotaxonomic and phenotypic data obtained, strain Z3-1 represents a novel species of the genus , for which the name sp. nov. is proposed with the type strain Z3-1 (=KCTC 62458=CCTCC AB 2017227).

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Keyword(s): Alexandrium tamarense , algae-associated bacteria , Nitratireductor alexandrii sp. nov , phycosphere microbiota and toxic marine dinoflagellate
Funding
This study was supported by the:
  • Special Fund for Scientific Innovation Strategy-construction of High Level Academy of Agriculture Science (Award R2020YJ-LJ001)
    • Principle Award Recipient: Xi Yang
  • Special Fund for Scientific Innovation Strategy-construction of High Level Academy of Agriculture Science (Award R2020YJ-LJ001)
    • Principle Award Recipient: Xi Yang
  • Presidential Foundation of Guangdong Academy of Agricultural Sciences, GAAS (Award 201813B, and 201918)
  • Natural Science Foundation of Zhejiang Province (Award LY18D060007)
    • Principle Award Recipient: Qiao Yang
  • National Natural Science Foundation of China (Award 41876114)
© 2020 The Authors
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2020-06-26
2024-04-25
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Nitratireductor alexandrii sp. nov., from phycosphere microbiota of toxic marine dinoflagellate Alexandrium tamarense
Int J Syst Evol Microbiol 70, 4390 (2020); https://doi.org/10.1099/ijsem.0.004302
/content/journal/ijsem/10.1099/ijsem.0.004302
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