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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 5

sp. nov., multiple polymer-degrading bacteria isolated from soil in China Free

Abstract

A short rod-shaped, Gram-stain-negative strain that can degrade multiple polymers was isolated from forest soil in China and designated as DSWY01. The results of 16S rRNA gene sequence analysis showed that this isolate shared high similarities with NBRC 102411 (99.3 %), NBRC 14162 (99.2%) and NBRC 13583 (99.0%). The results of phylogenetic analysis based on 16S rRNA gene sequence and multilocus sequence analysis (, , , and ) indicated that strain DSWY01 belongs to the genus and is a member of the group in an independent branch. The average nucleotide identity and digital DNA–DNA hybridization between the genome of strain DSWY01 and the genomes of other species (ANIb 77.72–89.65 %; GGDC 15.50–31.10 %) showed that the isolate represents a novel species. The DNA G+C content of strain DSWY01 was 63.67 mol%, and the major cellular fatty acids (>15 %) were a mixture of Cω7/Cω6 and C. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and two unidentified lipids, and the major quinone was CQ-10. The morphological, physiological and biochemical characteristics of the isolate were then compared with those of reference type strains. The isolate differed considerably from its closest relatives and is representative of a novel species of , for which the name sp. nov. is proposed. The type strain is DSWY01 (=DSM 106702=CCTCC AB 2018053).

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): multiple polymer-degrading , Pseudomonas hydrolytica and taxonomic position
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31870048)
    • Principle Award Recipient: Fan Li
© 2020 The Authors
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2020-04-03
2024-04-25
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Pseudomonas hydrolytica sp. nov., multiple polymer-degrading bacteria isolated from soil in China
Int J Syst Evol Microbiol 70, 3049 (2020); https://doi.org/10.1099/ijsem.0.004129
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