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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 67, Issue 12

sp. nov., isolated from the root surface of field-grown maize Free

Abstract

A Gram-positive-staining, aerobic, non-endospore-forming bacterial strain (JJ-59), isolated from a field-grown maize plant in Dunbar, Nebraska in 2014 was studied by a polyphasic approach. Based on 16S rRNA gene sequence similarity comparisons, strain JJ-59 was shown to be a member of the genus , most closely related to the type strains of (98.6 % 16S rRNA gene sequence similarity) and (97.8 %). For all other type strains of species of the genus lower 16S rRNA gene sequence similarities were obtained. DNA–DNA hybridization values of strain JJ-59 to the type strains of and were 26 % (reciprocal, 59 %) and 52 % (reciprocal, 59 %), respectively. Chemotaxonomic characteristics such as the presence of diaminopimelic acid in the peptidoglycan, the major quinone MK-7 and spermidine as the major polyamine were in agreement with the characteristics of the genus . Strain JJ-59 shared with its next related species the major lipids diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified aminophospholipid, but the presence/absence of certain lipids was clearly distinguishable. Major fatty acids of strain JJ-59 were anteiso-C, iso-C and iso-C, and the genomic G+C content is 47.2 mol%. Physiological and biochemical characteristics of strain JJ-59 were clearly different from the most closely related species of the genus . Thus, strain JJ-59 represents a novel species of the genus , for which the name sp. nov. is proposed, with JJ-59 (=DSM 103623=CIP 111179=LMG 29764) as the type strain.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): endophyte , Paenibacillus nebraskensis , plant associated , taxonomy and Zea mays
© 2017 IUMS
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2017-12-01
2024-04-19
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Paenibacillus nebraskensis sp. nov., isolated from the root surface of field-grown maize
Int J Syst Evol Microbiol 67, 4956 (2017); https://doi.org/10.1099/ijsem.0.002357
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