1887

Abstract

A novel strain, designated strain CU3-7, was isolated from faeces of a two-week-old baby. The isolate was Gram-staining-positive, anaerobic and rod-shaped. Results from 16S rRNA gene sequence analysis revealed that strain CU3-7 was phylogenetically affiliated with members of the genus . Strain CU3-7 showed the highest level of sequence similarity with KCTC 3216 (98.4 %), followed by KCTC 3425 (97.9 %). Analysis of sequences showed that strain CU3-7 was closely related to KCTC 3216 (94.0 %) and KCTC 3425 (92.5 %). The DNA–DNA hybridization values with the closely related strains were all below the cut-off value for species delineation, 17.0 % with KCTC 3425 and 14.9 % with KCTC 3216. Fructose-6-phosphate phosphoketolase activity was detected. The predominant cellular fatty acids were C (27.7 %), Cω9 (27.4 %) and Cω9 dimethylacetate (15.5 %). The DNA G+C content was 58.6 mol%. On the basis of polyphasic taxonomy, strain CU3-7 should be classified as the type strain of a novel species within the genus , for which the name sp. nov. is proposed ( = KACC 17904 = JCM 19861).

Funding
This study was supported by the:
  • Korea Food Research Institute (Award E0131901)
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2014-09-01
2024-03-29
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