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Abstract

A novel Gram-negative, aerobic, motile and rod-shaped bacterium was isolated from Qurugöl Lake located in a mountainous region near Tabriz city in the north-west of Iran. Growth occurred at pH 6–10 (optimum, pH 7±0.5) and at 10–40 °C (optimum, 30 °C). Strain RCRI4 was able to grow in the absence and presence of NaCl to 3 % (w/v). The major fatty acids were C, Cω7/Ciso3-OH, Cω8 and C. The G+C content of genomic DNA was 45.3 mol%. Based on the 16S rRNA and gene sequences, phylogenetic analyses indicated that strain RCRI4 associated with the genus , and closely related type strains include BLO6 (97.8 %), B11 (97.7 %), BA131 (97.5 %), CCUG 30811 (97.3 %) and MS1 (97.1 %). The level of DNA–DNA relatedness between strain RCRI4 and phylogenetically the closest related strains, BLO6 and BA131, was 9 and 14 %, respectively. On the basis of phenotypic, chemotaxonomic and phylogenetic results, it is suggested that strain RCRI4 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is RCRI4 ( = LMG 26473 = JCM 17275 = KCTC 23723).

Funding
This study was supported by the:
  • Rice and Citrus Research Institute (RCRI)
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2012-08-01
2024-04-19
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References

  1. Brettar I., Christen R., Höfle M. G. 2002; Rheinheimera baltica gen. nov., sp. nov., a blue-coloured bacterium isolated from the central Baltic sea. Int J Syst Evol Microbiol 52:1851–1857 [View Article][PubMed]
    [Google Scholar]
  2. Brettar I., Christen R., Höfle M. G. 2006; Rheinheimera perlucida sp. nov., a marine bacterium of the Gammaproteobacteria isolated from surface water of the central Baltic Sea. Int J Syst Evol Microbiol 56:2177–2183 [View Article][PubMed]
    [Google Scholar]
  3. Cashion P., Holder-Franklin M. A., McCully J., Franklin M. 1977; A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81:461–466 [View Article][PubMed]
    [Google Scholar]
  4. Chun J., Lee J. H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
    [Google Scholar]
  5. Corbin D. R., Grebenok R. J., Ohnmeiss T. E., Greenplate J. T., Purcell J. P. 2001; Expression and chloroplast targeting of cholesterol oxidase in transgenic tobacco plants. Plant Physiol 126:1116–1128 [View Article][PubMed]
    [Google Scholar]
  6. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [View Article]
    [Google Scholar]
  7. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. 1994 Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology;
    [Google Scholar]
  8. Gregersen T. 1978; Rapid method for distinction of Gram-negative from Gram-positive bacteria. Eur J Appl Microbiol Biotechnol 5:123–127 [View Article]
    [Google Scholar]
  9. Karlson U., Dwyer D. F., Hooper S. W., Moore E. R. B., Timmis K. N., Eltis L. D. 1993; Two independently regulated cytochromes P-450 in a Rhodococcus rhodochrous strain that degrades 2-ethoxyphenol and 4-methoxybenzoate. J Bacteriol 175:1467–1474[PubMed]
    [Google Scholar]
  10. Kim M. S., Roh S. W., Nam Y. D., Chang H. W., Kim K. H., Jung M. J., Choi J. H., Park E. J., Bae J. W. 2009; Alishewanella jeotgali sp. nov., isolated from traditional fermented food, and emended description of the genus Alishewanella . Int J Syst Evol Microbiol 59:2313–2316 [View Article][PubMed]
    [Google Scholar]
  11. Kim M. S., Jo S. K., Roh S. W., Bae J. W. 2010; Alishewanella agri sp. nov., isolated from landfill soil. Int J Syst Evol Microbiol 60:2199–2203 [View Article][PubMed]
    [Google Scholar]
  12. MacFaddin J. F. 2000 Biochemical Tests for Identification of Medical Bacteria New York: Lippincott Williams & Wilkins;
    [Google Scholar]
  13. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
    [Google Scholar]
  14. Roh S. W., Nam Y. D., Chang H. W., Kim K. H., Kim M. S., Oh H. M., Bae J. W. 2009; Alishewanella aestuarii sp. nov., isolated from tidal flat sediment, and emended description of the genus Alishewanella . Int J Syst Evol Microbiol 59:421–424 [View Article][PubMed]
    [Google Scholar]
  15. Skerman V. B. K. 1967 A Guide to the Identification of the Genera of Bacteria, 2nd edn. Baltimore: Williams & Wilkins;
    [Google Scholar]
  16. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [View Article]
    [Google Scholar]
  17. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [View Article]
    [Google Scholar]
  18. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. 2011; mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739 [View Article][PubMed]
    [Google Scholar]
  19. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  20. Vogel B. F., Venkateswaran K., Christensen H., Falsen E., Christiansen G., Gram L. 2000; Polyphasic taxonomic approach in the description of Alishewanella fetalis gen. nov., sp. nov., isolated from a human foetus. Int J Syst Evol Microbiol 50:1133–1142 [View Article][PubMed]
    [Google Scholar]
  21. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]
    [Google Scholar]
  22. Yamamoto S., Harayama S. 1995; PCR amplification and direct sequencing of gyrB genes with universal primers and their application to the detection and taxonomic analysis of Pseudomonas putida strains. Appl Environ Microbiol 61:1104–1109[PubMed]
    [Google Scholar]
  23. Yoon J. H., Kang S. J., Oh T. K. 2007; Donghicola eburneus gen. nov., sp. nov., isolated from seawater of the East Sea in Korea. Int J Syst Evol Microbiol 57:73–76 [View Article][PubMed]
    [Google Scholar]
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