1887

Abstract

A novel endophytic actinomycete, strain YIM 65646, was isolated from the roots of L. collected from Yunnan Province, south-west China. Growth was observed in the temperature range 10–40 °C (optimum 20–28 °C) and at pH 6.0–9.0 (optimum pH 7.0). The organism formed well-developed, branched substrate mycelia, but aerial mycelium was not produced. Phenotypic characterization and 16S rRNA gene sequence analysis indicated that strain YIM 65646 belonged to the family (sharing ≤93.6 % sequence similarity with members of this family) and formed a distinct clade in the phylogenetic tree. The strain contained -diaminopimelic acid in the cell wall and mannose, ribose, galactose and glucose in whole-cell hydrolysates. The major menaquinones were MK-10(H), MK-10(H), MK-8(H), MK-9(H) and MK-10(H). The major fatty acids were iso-C, anteiso-C, anteiso-C, iso-C and iso-C. The DNA G+C content of strain YIM 65646 was 70.0 mol%. On the basis of morphological and chemotaxonomic properties and phylogenetic analysis based on 16S rRNA gene sequence data, it is proposed that this strain should be classified in a novel genus and species, gen. nov., sp. nov., in the family . The type strain is YIM 65646 ( = CCTCC AA 209041  = DSM 45386).

Funding
This study was supported by the:
  • National Basic Research Program of China (Award 2010CB833801)
  • National Natural Science Foundation of China (Award U0932601)
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2011-12-01
2024-03-29
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