1887

Abstract

For survival, pathogenic organisms such as must possess an efficient mechanism for acquiring iron in the iron-restricted environment of the human body. can use iron from a variety of sources found within the host. However, it is not clear how biologically active ferrous iron is obtained from these sources. One strategy adopted by some organisms is to reduce iron extracellularly and then specifically transport the ferrous iron into the cell. We have shown that clinical isolates of do have a cell-associated ferric-reductase activity. The determination of ferric-reductase activity of cells growing exponentially in either low- or high-iron media over a period of time indicated that reductase activity is induced when in low-iron conditions. Moreover, we have demonstrated that reductase activity is also regulated in response to the growth phase of the culture, with induction occurring upon exit from stationary phase and maximal levels being reached in early exponential stage irrespective of the iron content of the medium. These results suggest that reductase activity is regulated in a very similar manner to the ferric-reductase. Iron reduction and uptake in are closely connected to copper reduction, and possibly copper uptake. In this report we show that iron and copper reduction also appear to be linked in The ferric-reductase activity is negatively regulated by copper. Moreover, quantitative cupric-reductase assays indicated that is capable of reducing copper and that this cupric-reductase activity is negatively regulated by both iron and copper. This is the first report that has an iron- and copper-mediated ferric-reductase activity.

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1996-03-01
2024-03-28
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References

  1. Askwith C., Eide D., Van Ho A., Bernard P. S., Li L., Davis-Kaplan S., , Sipe D. M., Kaplan J. 1994; The FET3 gene of S cerevisiae encodes a multicopper oxidase required for ferrous iron uptake. Cell 76:403–410
    [Google Scholar]
  2. Dancis A., Klasner R. D., Hinnebusch A. G., Barriocanal J. G. 1990; Genetic evidence that ferric reductase is required for iron uptake in Saccharomyces cerevisiae . Mol Cell Biol 10:2294–2301
    [Google Scholar]
  3. Dancis A., Yuan D. S., Haile D., Askwith C., Eide D., Moehle G, Kaplan J., Klausner R. D. 1994; Molecular characterization of a copper transport protein in S. cerevisiae: an unexpected role for copper in iron transport. Cell 76:393–402
    [Google Scholar]
  4. Eide D., Davis-Kaplan S., , Jordan I., Sipe D., Kaplan J. 1992; Regulation of iron uptake in Saccharomyces cerevisiae The ferri-reductase and Fe(II) transporter are regulated independently. J Biol Chem 267:20774–20781
    [Google Scholar]
  5. Evans S. L., Arceneaux J. E. L., Byers B. R., Martin M. E., Aranha N. 1986; Ferrous iron transport in Streptococcus mutans . J Bacteriol 168:1096–1099
    [Google Scholar]
  6. Georgatsou E., Alexandraki D. 1994; Two distinctly regulated genes are required for ferric reduction, the first step of iron uptake in Saccharomyces cerevisiae . Mol Cell Biol 14:3065–3073
    [Google Scholar]
  7. Georgatsou E., Mavrogiannis L., Frangiadakis G., Klinakis A., Alexandraki D. 1995; The Saccharomyces cerevisiae ferric reductase genes FRE1 and FRE2 are distinctly regulated by iron, copper and stress related factors. Yeast 11:S155
    [Google Scholar]
  8. Hassett R., Kosman D. J. 1995; Evidence for Cu(I) reduction as a component of copper uptake by S. cerevisiae . J Biol Chem 270:128–134
    [Google Scholar]
  9. Ismail A., Bedell G. W., Lupan D. M. 1985; Siderophore production by the pathogenic yeast, Candida albicans . Biochem Biophys Res Commun 130:885–891
    [Google Scholar]
  10. Johnson W., Varner L., Poch M. 1991; Acquisition of iron by Leeionella pneumophila: role of iron-reductase. Infect Immun 59:2376–2381
    [Google Scholar]
  11. Jungmann J., Reins H.-A., Lee J., Romeo A., Hassett R., Kosman D., Jentsch S. 1993; MAC1 a nuclear regulatory protein related to Cu-dependent transcription factors is involved in Cu/Fe utilization and stress resistance in yeast. EMBO J 12:5051–5056
    [Google Scholar]
  12. Kerridge D. 1993; Fungal dimorphism: a sideways look. Dimorphic Fungi in Biology and Medicine3–10 Bossche H. V., Odds F. C., Kerridge D. New York: Plenum Press;
    [Google Scholar]
  13. Lesuisse E., Labbe P. 1989; Reductive and non-reductive mechanisms of iron assimilation by the yeast Saccharomyces cerevisiae . J Gen Microbiol 135:257–263
    [Google Scholar]
  14. Lesuisse E., Raguzzi F., Crichton R. R. 1987; Iron uptake by the yeast Saccharomyces cerevisiae: involvement of a reduction step. J Gen Microbiol 133:3229–3236
    [Google Scholar]
  15. Lesuisse E., Crichton R. R., Labbe P. 1990; Iron reductases in the yeast Saccharomyces cerevisiae . Biochim Biophys Acta 1038:253–259
    [Google Scholar]
  16. Lesuisse E., Simon M., Klein R., Labbe P. 1992; Excretion of anthranilate and 3-hydroxyanthranilate by Saccharomyces cerevisiae: relationship to iron metabolism. J Gen Microbiol 138:85–89
    [Google Scholar]
  17. Minnick A. A., Eizember L. E., McKee J. A., Dolence E. K., Miller M. J. 1991; Bioassay for siderophore utilization by Candida albicans . Anal Biochem 194:223–229
    [Google Scholar]
  18. Moors M. A., Stull T. L., Blank K. J., Buckley H. R., Mosser D. M. 1992; A role for complement receptor-like molecules in iron acquisition by Candida albicans . J Exp Med 175:1643–1651
    [Google Scholar]
  19. Neilands J. B., Konopka K., Schwyn B., Coy M., Francis R. I., Paw B. H., Bagg A. 1987; Comparative biochemistry of microbial iron assimilation. Iron Transport in Microbes, Plants and Animals3–34 Winkelmann G., van der Helm D., Neilands J. B. Weinheim: VCH;
    [Google Scholar]
  20. Odds F. C. 1987; Candida infections: an overview. Crit Rev Microbiol 15:1–5
    [Google Scholar]
  21. Roman D. G., Dancis A., Anderson G. J., Klausner R. D. 1993; The fission yeast ferric reductase gene frp1 + is required for ferric iron uptake and encodes a protein that is homologous to the gp91-phox subunit of the human NADPH phagocyte oxidoreductase. Mol Cell Biol 13:4342–4350
    [Google Scholar]
  22. Ryley J. F., Ryley N. G. 1990; Candida albicans – do mycelia matter?. J Med Vet Mycol 23:225
    [Google Scholar]
  23. Sherman F., Fink G. R., Hicks J. B. 1986 Laboratory Course Manual for Methods in Yeast Genetics Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  24. Sudbery P. 1994; The won-Saccharomyces yeasts. Yeast 10:1707–1726
    [Google Scholar]
  25. Sweet S. P., Douglas L. J. 1991a; Effect of iron concentrations on siderophore synthesis and pigment production of Candida albicans . FEMS Microbiol Lett 80:87–91
    [Google Scholar]
  26. Sweet S. P., Douglas L. J. 1991b; Effect of iron deprivation on surface composition and virulence determinants of Candida albicans . J Gen Microbiol 137:859–865
    [Google Scholar]
  27. Valenti P., Visca P., Antonini G., Orsi N. 1986; Interaction between lactoferrin and ovotransferrin and Candida cells. FEMS Lett 33:271–275
    [Google Scholar]
  28. Wickerham L. J. 1951; Taxonomy of yeast. US Dep Agric Tech Bull 1029:11–56
    [Google Scholar]
  29. Wooldridge K. G., Williams P. H. 1993; Iron uptake mechanisms of pathogenic bacteria. FEMS Microbiol Lett 12:325–348
    [Google Scholar]
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