1887

Abstract

Five of eight strains of and one of 13 strains of were found to harbour DNA plasmids, pSB1 and pSB2 plasmids were isolated from strains IFO 0488 and IFO 1047, respectively, and pSB3 and pSB4 from strain IFO 1730. All four plasmids resemble 2-μm DNA of in that (i) their molecular sizes are about 6 kb, (ii) each molecule possesses a pair of inverted repeats, (iii) they exist as a mixture of two isomers and (iv) their copy numbers in the native host are similar. None of them showed homology with 2-μm DNA or with each other by Southern hybridization under moderately stringent conditions, but pSB4 hybridized with the pSR1 DNA, which was found previously in a strain of Each of the pSB plasmids has DNA sequence(s) effective for autonomous replication in In pSB3 and pSB4 showed intramolecular recombination but neither supported isomerization of 2-μm DNA.

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/content/journal/micro/10.1099/00221287-130-10-2527
1984-10-01
2024-03-28
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References

  1. Beggs J.D. 1978; Transformation of yeast by a replicating hybrid plasmid. Nature: London; 275104–109
    [Google Scholar]
  2. Birnboim H.C., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7:1513–1523
    [Google Scholar]
  3. Botstein D., Falco S.C., Stewart S.E., Brennan M., Scherer S., Stinchomb D.T., Struhl K., Davis R.W. 1979; Sterile host yeasts (SHY): aeukaryotic system of biological containment for recombinant DNA experiments. Gene 8:17–24
    [Google Scholar]
  4. Broach J.R. 1981; The yeast plasmid 2 p circle. In The Molecular Biology of the Yeast Saccharomyces. Life Cycle and Inheritance pp. 445–470 Jones E.W., Strathern J.N., Broach J.R. Edited by Cold Spring Harbor, New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  5. Broach J.R., Guarascio V.R.G., Jayaram M. 1982; Recombination within the yeast plasmid 2 p circle is site-specific. Cell 29:227–234
    [Google Scholar]
  6. Cameron J.R., Philippsen P., Davis R.W. 1977; Analysis of chromosomal integration and deletion of yeast plasmids. Nucleic Acids Research 4:1429–1448
    [Google Scholar]
  7. Davis R.W., Simon M., Davidson N. 1971; Electron microscope heteroduplex methods for mapping regions of base sequence homology in nucleic acids. Methods in Enzymology 21D:413–428
    [Google Scholar]
  8. Gunge N., Tamaru A., Ozawa F., Sakaguchi K. 1981; Isolation and characterization of linear deoxyribonucleic acid plasmids from Kluyveromyces lactis and the plasmid-associated killer character. Journal of Bacteriology 145:382–390
    [Google Scholar]
  9. Hartley J.L., Donelson J.E. 1980; Nucleotide sequence of the yeast plasmid. Nature: London; 286860–864
    [Google Scholar]
  10. Messing J., Crea R., Seeburg P.H. 1981; A system for shotgun DNA sequencing. Nucleic Acids Research 9:309–321
    [Google Scholar]
  11. Rigby P.W, Rhodes C., Berg P. 1977; Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. Journal of Molecular Biology 113:237–251
    [Google Scholar]
  12. Southern E.M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  13. Sutcliffe J.G. 1978; Complete nucleotide sequence of the Escherichia coli plasmid pBR322. Cold Spring Harbor Symposia on Quantitative Biology 43:77–90
    [Google Scholar]
  14. Szybalski E.M., Szybalski W. 1979; A comprehensive molecular map of bacteriophage lambda. Gene 7:217–270
    [Google Scholar]
  15. Toh-E A., Wickener R.B. 1981; Curing of the 2p DNA plasmid from Saccharomyces cerevisiae. Journal of Bacteriology 145:1421–1424
    [Google Scholar]
  16. Toh-E A., Tada S., Oshima Y. 1982; 2-pm DNA- like plasmids in the osmophilic haploid yeast Saccharomyces rouxii. Journal of Bacteriology 151:1380–1390'
    [Google Scholar]
  17. Van DER WALT J.P. 1970; Saccharomyces Meyen emend. Reess. In The Yeasts, a Taxonomic Study pp. 555–715 Lodder J. Edited by Amsterdam: North- Holland Publishing Co;
    [Google Scholar]
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