1887

Abstract

Exopolysaccharides and lipopolysaccharides were prepared from a number of myxobacterial strains representing the more complex types. The exopolysaccharides were isolated from fruiting bodies and from liquid and solid cultures. The polysaccharides secreted by the bacillary forms in solid or liquid media closely resembled the material obtained from fruiting bodies, the monosaccharides present being in the same approximate molar ratios. Many of the sugars present in the exopolysaccharides were also detected in the lipopoly-saccharides, suggesting an economic use of sugar nucleotide synthetic systems. Several, but not all, lipopolysaccharides contained material resembling 3-O-methylxylose in its chromatographic mobility. In addition, a faster-moving spot, as yet unidentified, was noted in some hydrolysates. The commonest monosaccharide components of the lipo-polysaccharide were rhamnose, mannose, glucose and galactose. Small quantities of amino sugars, particularly glucosamine and galactosamine, were also detected.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-111-1-211
1979-03-01
2024-04-23
Loading full text...

Full text loading...

/deliver/fulltext/micro/111/1/mic-111-1-211.html?itemId=/content/journal/micro/10.1099/00221287-111-1-211&mimeType=html&fmt=ahah

References

  1. BROCKMAN E. R., ToDD R. L. 1974; Fruiting myxobacteria as viewed with a scanning electron microscope. International Journal of Systematic Bacteriology 24:118–124
    [Google Scholar]
  2. DUBOIS M, GILLES K. A., HAMILTON J. K., REBERS P. A., SMITH F. 1956; Colorimetric method for determination of sugars and related substances.. Analytical Chemistry 28:350–356
    [Google Scholar]
  3. GERTH K., REJCHENBACH H. 1978; Induction of myxospore formation in Stigmatella aurantiaca (Myxobacterales). I. General characterization of the system.. Archives of Microbiology 117:173–182
    [Google Scholar]
  4. GRILIONE P. L., PANGBORN J. 1975; Scanningelectron microscopy of fruiting body formation by myxobacteri. Journal of Bacteriology 124:1558–1565
    [Google Scholar]
  5. REICHENBACH H, DWORKIN M. 1969; Studies on Stigmatella aurant iaca (M yxobac terales) .. Journal of General Microbiology 58:3–14
    [Google Scholar]
  6. SUTHERLAND I. W., MACKENZIE C. L. 1977; Glucan common to the microcyst walls of cystforming bacteria.. Journal of Bccteriology 129:599–605
    [Google Scholar]
  7. SUTHERLD L. W., SMITH M. L. 1973; The lipopolysaccharides of fruiting and non-fruiting myxobacteri. Journal of General Microbiology 74:259–266
    [Google Scholar]
  8. SUTHERLAND I.W., THOMSON S. 1975; parison of polysaccharides produced by Myxococcus strains. Journal of General Microbiology 89:124–132
    [Google Scholar]
  9. THARANATHAN R. N.,, WECKESSER J. 1978; Location of 0-methyl sugars in antigenic (lipo-)polysaccharides of photosynthetic bacteria and cyanobacteria.. Biochemical Journal 171:403–408
    [Google Scholar]
  10. WECKESSER J., ROSENFELDER G.,, MAYER H., LUDERITZ O. 1971; The identification of 3-0-methyl-D-xylose and 3-O-methyl-~-xylosea s constituents of the lipop lysaccharides of Myxococcus fulvus and Rhodopseudomonas viridis respectively. International Journal of Systematic Bacteriology 24:112–115
    [Google Scholar]
  11. WECKESSER J., DREWS G., ROPPEL J., MAYER H., FROMME I. 1974; he lipopolysaccharides (0-antigens) of Rhodopseudomonas viridis.. Archivesof Microbiology 101:245–124
    [Google Scholar]
  12. WHITE D. Myxospores of Myxococcus xanthusMyxospores of Myxococcus xanthu. In Gerhardt P., Costilow R. N., Sadoff H. L. pp. Spores Washington: American Society for Microbiology; IV44–51 (1975)
    [Google Scholar]
  13. WILKINSON S.G. Composition and structure of bacterial lipopolysaccharides. In Sutherland I. W. (editors) Surface Carbohydrates of the Prokaryotic Cell, London: Academic Press;97–175 1977
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-111-1-211
Loading
/content/journal/micro/10.1099/00221287-111-1-211
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error