1887

Abstract

Tobacco rattle virus (TRV) isolate PPK20 is transmitted by nematodes. The factor(s) determining vector transmissibility has been shown to be located on TRV RNA 2. Sequence determination revealed that PPK20 RNA 2 contains three open reading frames encoding the coat protein (cp) and proteins with molecular masses of 29.4 and 32.8 kDa. The 29.4 and 32.8 kDa protein-coding genes showed no significant sequence similarity to any other known tobravirus gene. A full-length cDNA of PPK20 RNA 2 cloned between the 35S promoter and terminator infected plants when co-inoculated with PPK20 RNA 1. Deletions in the reading frames of the 29.4 and 32.8 kDa proteins revealed that these sequences are dispensable for replication of PPK20 RNA 2 in plants. Subgenomic RNAs for translation of cp and the putative 29.4 and 32.8 kDa proteins were detected in infected leaves. The possible role of PPK20 RNA 2 nonstructural genes in TRV vector transmission is discussed.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-76-11-2847
1995-11-01
2024-04-16
Loading full text...

Full text loading...

/deliver/fulltext/jgv/76/11/JV0760112847.html?itemId=/content/journal/jgv/10.1099/0022-1317-76-11-2847&mimeType=html&fmt=ahah

References

  1. Angenent G. C., Linthorst H. J. M., Van Belkum A. F., Cornelis-sen B. J. C., Bol J. F. 1986; RNA 2 of tobacco rattle virus strain TCM encodes an unexpected gene. Nucleic Acids Research 14:4673–4682
    [Google Scholar]
  2. Angenent G. C., Posthumus E., Brederode F. T., Bol J. F. 1989; Genome structure of tobacco rattle virus strain PLB: further evidence on the occurrence of RNA recombination among tobra viruses. Virology 171:271–274
    [Google Scholar]
  3. Armour S. L., Melcher U., Pirone T. P., Lyttle D. J., Essenberg R. C. 1983; Helper components for aphid transmission encoded by region II of cauliflower mosaic virus DNA. Virology 129:25–30
    [Google Scholar]
  4. Bergh S. T., Koziel M. G., Huang S.-C., Thomas R. A., Gilley D. P., Siegel A. 1985; The nucleotide sequence of tobacco rattle virus RNA 2 (CAM strain). Nucleic Acids Research 13:8507–8518
    [Google Scholar]
  5. Brault V., Van Den Heuvel J. F. J. M., Verbeek M., Ziegler-Graff V., Reutenauer A., Herrbach E., Garaud J.-C., Guilley H., Richards K., Jonard G. 1995; Aphid transmission of beet western yellows luteovirus requires the minor capsid read-through protein P74. EMBO Journal 14:650–659
    [Google Scholar]
  6. Brown D. J. F., Ploeg A. T., Robinson D. J. 1989; The association between serotypes of tobraviruses and Trichodorus and Paratrichodorus species. EPPO Bulletin 19:611–617
    [Google Scholar]
  7. Cornelissen B. J. C., Linthorst H. J. M., Brederode F. T., Bol J. F. 1986; Analysis of the genome structure of tobacco rattle virus strain PSG. Nucleic Acids Research 14:2157–2169
    [Google Scholar]
  8. Goulden M. G., Lomonossoff G. P., Davies J. W., Wood K. R. 1990; The complete nucleotide of PEBV RNA 2 reveals the presence of a novel open reading frame and provides insights into the structure of tobraviral subgenomic promoters. Nucleic Acids Re- search 18:4507–4512
    [Google Scholar]
  9. Hamilton W. D. O., Boccara M., Robinson D. J., Baulcombe D. C. 1987; The complete nucleotide sequence of tobacco rattle virus RNA-1. Journal of General Virology 68:2563–2575
    [Google Scholar]
  10. Johnsen M. G., Rasmussen O. F., Albrechtsen M., Borkhardt B. 1991; In vivo expression of the 29000 M r protein from RNA-2 of pea early browning tobravirus. Journal of General Virology 72:1223–1227
    [Google Scholar]
  11. Macfarlane S. A., Brown D. J. F. 1995; Sequence comparison of RNA2 of nematode-transmissible and nematode non transmissible isolates of pea early browning virus suggests that the gene encoding the 29 kDa protein may be involved in nematode transmission. Journal of General Virology 76:1299–1304
    [Google Scholar]
  12. Macfarlane S. A., Taylor S. C., King D. I., Hughes G., Davies J. W. 1989; Pea early browning virus RNA 1 encodes four polypeptides including a putative zinc-finger protein. Nucleic Acids Research 17:2245–2260
    [Google Scholar]
  13. Mayo M. A., Brierley K. M., Goodman B. A. 1993; Developments in the understanding of the particle structure of tobraviruses. Biochemie 75:639–644
    [Google Scholar]
  14. Pirone T. P., Thornbury D. W. 1984; The involvement of a helper component in nonpersistent transmission of plant viruses by aphids. Microbiological Science 1:190–193
    [Google Scholar]
  15. Ploeg A. T. Brown F D. J., Robinson D. J. 1989; Transmission of tobraviruses by trichodorid nematodes. EPPO Bulletin 19605–610
    [Google Scholar]
  16. Ploeg A. T., Brown D. J. F., Robinson D. J. 1992; The association between species of Trichodorus and Paratrichodorus vector nematodes and serotypes of tobacco rattle virus. Annals of Applied Biology 121:619–630
    [Google Scholar]
  17. Ploeg A. T., Robinson D. J., Brown D. J. F. 1993; RNA 2 of tobacco rattle virus encodes the determinants of transmissibility by trichodorid nematodes. Journal of General Virology 74:1463–1466
    [Google Scholar]
  18. Yamaya J., Yoshioka M., Meshi T., Okada Y., Ohno T. 1988; Expression of tobacco mosaic virus RNA in transgenic plants. Molecular and General Genetics 211:520–525
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-76-11-2847
Loading
/content/journal/jgv/10.1099/0022-1317-76-11-2847
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error