1887

Abstract

SUMMARY

We report a simple method for the isolation of the measles virus glycoproteins, and their subsequent incorporation into artificial lipid bilayers. The two viral glycoprotins, HA and F, were isolated in preparative amounts from disrupted purified virus by lentil lectin affinity chromatography. The proteins were reconstituted into single bilayer lipid vesicles by: (i) exchanging the non-dialysable detergent Nonidet P40 (NP40) for a dialysable one, octylglucoside, while the proteins were immobilized on the lectin column and (ii) co-dialysis of the eluted glycoproteins in octylglucoside with phosphatidylcholine. The resultant ‘virosomes’ had visible ‘spikes’ and possessed haemagglutinating activity. These measles virosomes should provide a useful reagent for studying immune responses to measles virus, independent of the immunosuppressive effects of the whole virus.

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1981-05-01
2024-03-29
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References

  1. Ault K. A., Weiner H. L. 1979; Natural killing of measles infected cells by human lymphocytes. Journal of Immunology 122:2611–2616
    [Google Scholar]
  2. Finberg R., Mischer M., Burakoff S. J. 1978; Introduction of virus specific cytotoxic T lymphocytes with isolated membrane proteins. Journal of Experimental Medicine 148:1620–1627
    [Google Scholar]
  3. Fujinami R. S., Sissons J. G. P., Oldstone M. B. A. 1980; Characterization and turnover of measles virus polypeptides expressed on the surface of infected cells. Journal of Immunology (in press)
    [Google Scholar]
  4. Graves M. C., Silver S. M., Choppin P. W. 1978; Measles virus polypeptide synthesis in infected cells. Virology 86:254–263
    [Google Scholar]
  5. Helenius A., Simons K. 1975; Solubilization of membranes by detergents. Biochimica et Biophysica Acta 415:29–79
    [Google Scholar]
  6. Helenius A., Fries E., Karienbeck J. 1977; Reconstitution of Semliki Forest virus membrane. Journal of Cell Biology 15:880–886
    [Google Scholar]
  7. Hsu M., Scheid A., Choppin P. W. 1979; Reconstitution of membranes with individual paramyxovirus glycoproteins and phospholipid in cholate solution. Virology 95:476–491
    [Google Scholar]
  8. Huddlestone J. R., Lampert P., Oldstone M. B. A. 1979; Measles virus infection of T lymphocyte subpopulation. In Virus-Lymphocyte Interactions: Implications for Disease Edited by Proffitt M. R. New York and Amsterdam: Elsevier/North-Holland;
    [Google Scholar]
  9. Huddlestone J. R., Lampert P. W., Oldstone M. B. A. 1980; Virus-lymphocyte interactions: infection of T8 and T/i subsets by measles virus. Clinical Immunology and Immunopathology 15:502
    [Google Scholar]
  10. Joseph B. S., Lampert P. W., Oldstone M. B. A. 1975; Replication and persistence of measles virus in defined subpopulations of human leukocytes. Journal of Virology 16:1638–1649
    [Google Scholar]
  11. Koszinowski U. H., Allen H., Bething M. J., Waterfield M. D., Klenk H. D. 1980; Recognition of viral glycoproteins by influenza A-specific cross-reactive cytolytic T lymphocytes. Journal of Experimental Medicine 151:45–958
    [Google Scholar]
  12. Kreth H. W., Ter meulen V., Eckert G. 1979; Demonstration of HLA restricted killer cells in patients with acute measles. Medical Microbiology and Immunology 165:203–214
    [Google Scholar]
  13. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  14. Loh D., Ross A. H., Hale A. H., Baltimore D., Eisen H. N. 1978; Synthetic phospholipid vesicles containing a purified viral antigen and cell membrane proteins stimulate the development of cytotoxic T lymphocytes. Journal of Experimental Medicine 150:1067–1074
    [Google Scholar]
  15. Lucas C. J., Vbels-Postma J. C., Rezee A., Galana J. M. D. 1978; Activation of measles virus from latently infected human lymphocytes. Journal of Experimental Medicine 148:940–952
    [Google Scholar]
  16. Morein B., Barz D., Koszinowski U., Schirrmacher V. 1979; Integration of a virus membrane protein into the lipid bilayer of target cells as a prerequisite for immune cytolysis. Journal of Experimental Medicine 150:1383–1398
    [Google Scholar]
  17. Morgan E. M., Rapp F. 1977; Measles virus and its associated diseases. Bacteriological Review 41:636–666
    [Google Scholar]
  18. Norrby E. 1978; Viral antibodies in multiple sclerosis. Progress in Medical Virology 24:1–39
    [Google Scholar]
  19. Oldstone M. B. A., Fujinami R. S., Lampert P. 1980; Membrane and cytoplasmic changes in virus-infected cells induced by interactions of antiviral antibody with surface viral antigen. Progress in Medical Virology 26:45–93
    [Google Scholar]
  20. Pagano R. E., Sandra A., Takeichi M. 1978; Interactions of phospholipid vesicles with mammalian cells. Annals of the New York Academy of Sciences 308:185–199
    [Google Scholar]
  21. Perrin L. H., Joseph B. S., Cooper N. R., Oldstone M. B. A. 1976; Mechanism of injury of virus infected cells by antiviral antibody and complement: participation of IgG, F(ab′)2 and the alternative complement pathway. Journal of Experimenlal Medicine 143:1027
    [Google Scholar]
  22. Perrin L. H., Tishon A. J., Oldstone M. B. A. 1977; Immunologic injury in measles virus infections. III.Presence and characterization of human cytotoxic lymphocytes. Journal of Immunology 118:282–290
    [Google Scholar]
  23. Sissons J. G. P., Schreiber R. D., Perrin L. H., Cooper N. R., Oldstone M. B. A., Muller-Eberhard H. J. 1979; Lysis of measles virus infected cells by the purified cytolytic alternative complement pathway and antibody. Journal of Experimental Medicine 150:45–454
    [Google Scholar]
  24. Sissons J. G. P., Oldstone M. B. A., Schreiber R. D. 1980; Antibody independent activation of the alternative complement pathway by measles virus infected cells. Proceedings of the National Academy of Sciences of the United States of America 77:559–562
    [Google Scholar]
  25. Ter Meulenm V., Hall W. W. 1978; Slow virus infection of the nervous system: virological, immunological and pathogenetic considerations. Journal of General Virology 41:1–25
    [Google Scholar]
  26. Wright L. L., Levy N. L. 1979; Generation on infected fibroblasts of human T and non T lymphocytes with specific cytotoxicity, influenced by histocompatibility, against measles virus infected cells. Journal of Immunology 122:2379–2387
    [Google Scholar]
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